Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (testis)
Permeabilization
Yes - 0.05 Tween20
Specification
testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBST
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
GDNF is expressed in Sertoli cells in the mouse testis. I also stained GATA4, a marker of Sertoli cells (mouse anti-GATA4 antibody from Santa cruz, primary antibody was detected by anti-mouse Alexa 488).
Abcam response
We recommend including a fixation step with acetone/methanol (1:1).
Fixations with acetone counteract the protein crosslinking effect of PFA.
Contact our scientific support team (technical@abcam.com) for further information of troubleshooting PFA fixed frozen sections.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 12 2016