Anti-GDF7 抗体 [EPR16000] (ab189928)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16000] to GDF7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-GDF7 antibody [EPR16000]
GDF7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16000] to GDF7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Human fetal brain, C6 and Neuro-2a cell lysates; Human adenocarcinoma of colon and rat brain tissues, U87-MG cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16000 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab189928の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
|
|
IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/50.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50. |
ターゲット情報
-
機能
May play an active role in the motor area of the primate neocortex. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 151449 Human
- Entrez Gene: 238057 Mouse
- Entrez Gene: 252833 Rat
- Omim: 604651 Human
- SwissProt: Q7Z4P5 Human
- SwissProt: P43029 Mouse
- Unigene: 447688 Human
- Unigene: 271308 Mouse
-
別名
- bmp12 antibody
- bone morphogenetic protein 12 antibody
- GDF-7 antibody
see all
画像
-
Immunocytochemical analysis of 4% paraformaldehyde fixed U87-MG cells labeling GDF7 with ab189928 at 1/50 dilution, and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 as a secondary antibody. Counterstained with DAPI.
-
Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/10000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-GDF7 antibody [EPR16000] (ab189928) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa -
Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon tissue labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab189928 は 1 報の論文で使用されています。
- Fu W et al. Mesenchymal Behavior of the Endothelium Promoted by SMAD6 Downregulation Is Associated With Brain Arteriovenous Malformation Microhemorrhage. Stroke 51:2197-2207 (2020). PubMed: 32486965