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Anti-GCNT1 抗体 (ab102665)

Reviews (2)Q&A (1)References (1)
Western blot - Anti-GCNT1 antibody (ab102665)
  • Western blot - Anti-GCNT1 antibody (ab102665)

Key features and details

  • Rabbit polyclonal to GCNT1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-GCNT1 antibody

  • 製品の詳細

    Rabbit polyclonal to GCNT1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-428 of Human GCNT1 (NP_001481.2).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab102665の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF (1)
Use at an assay dependent concentration.
特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Forms critical branches in O-glycans.

  • 組織特異性

    Highly expressed in activated T-lymphocytes and myeloid cells.

  • パスウェイ

    Protein modification; protein glycosylation.

  • 配列類似性

    Belongs to the glycosyltransferase 14 family.

  • 細胞内局在

    Golgi apparatus membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 3-galactosyl-O-glycosyl-glycoprotein beta-1 antibody
      • 6-N-acetylglucosaminyltransferase antibody
      • Beta-1 antibody
      • Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase antibody
      • C2GNT antibody
      • C2GNT L antibody
      • C2GNT1 antibody
      • C2GnTL antibody
      • Core 2 branching enzyme antibody
      • Core 2 GNT antibody
      • Core 2-branching enzyme antibody
      • Core2 GlcNAc transferase antibody
      • Core2-GlcNAc-transferase antibody
      • GCNT 1 antibody
      • GCNT1 antibody
      • GCNT1_HUMAN antibody
      • glucosaminyl (N-acetyl) transferase 1 core 2 antibody
      • NACGT2 antibody
      see all

    画像

    • Western blot - Anti-GCNT1 antibody (ab102665)
      Western blot - Anti-GCNT1 antibody (ab102665)
      Anti-GCNT1 antibody (ab102665) at 1/500 dilution + Human Pancreas Tissue Lysate at 50 µg

      Predicted band size: 50 kDa

    • Western blot - Anti-GCNT1 antibody (ab102665)
      Western blot - Anti-GCNT1 antibody (ab102665)
      All lanes : Anti-GCNT1 antibody (ab102665) at 1/500 dilution

      Lane 1 : GCNT1 transfected 293T cell line
      Lane 2 : Non-transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 50 kDa

    参考文献 (1)

    ab102665 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab102665 は 1 報の論文で使用されています。

    • Nolz JC & Harty JT IL-15 regulates memory CD8+ T cell O-glycan synthesis and affects trafficking. J Clin Invest 124:1013-26 (2014). WB ; Mouse . PubMed: 24509081

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-GCNT1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Cell (Mouse tracheal tissue)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Mouse tracheal tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 Jan 20 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GCNT1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (KG1a)
    Specification
    KG1a
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 Apr 26 2012

    Question

    Would like to submit Abreview for this antibody used in ICC/IF.

    Read More
    Answer

    DISCOUNT CODE: ***
    Expiration date: August 25, 2012

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab102665 in ICC/IFand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com