Anti-GCLC 抗体 [EP13475] (ab190685)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP13475] to GCLC
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GCLC antibody [EP13475]
GCLC 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP13475] to GCLC -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: NIH/3T3, Jurkat and C6 whole cell lysate. IP: Jurkat whole cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP13475 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab190685の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (7) |
1/20000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
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IP |
1/20.
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特記事項 |
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WB
1/20000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
IP
1/20. |
ターゲット情報
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パスウェイ
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
関連疾患
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. -
配列類似性
Belongs to the glutamate--cysteine ligase type 3 family. - Information by UniProt
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参照データベース
- Entrez Gene: 2729 Human
- Entrez Gene: 14629 Mouse
- Entrez Gene: 25283 Rat
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P97494 Mouse
- SwissProt: P19468 Rat
- Unigene: 654465 Human
see all -
別名
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma glutamylcysteine synthetase antibody
see all
画像
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ab190685 at 1/20 dilution immunoprecipitating GCLC in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+): Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab190685 in Jurkat whole cell lysate
For western blotting, ab227648 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/50000 dilution were used.Blocking and diluting buffer: 5% NFDM /TBST.
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Anti-GCLC antibody [EP13475] (ab190685) at 1/2000 dilution + NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 180 seconds
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All lanes : Anti-GCLC antibody [EP13475] (ab190685) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaBlocking/diluting buffer and concentation: 5% NFDM/TBST
Exposure time lane 1: 80 seconds
Exposure time lane 2: 160 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (38)
ab190685 は 38 報の論文で使用されています。
- von Mässenhausen A et al. Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion. Sci Adv 8:eabl8920 (2022). PubMed: 35108055
- Laoukili J et al. Peritoneal metastases from colorectal cancer belong to Consensus Molecular Subtype 4 and are sensitised to oxaliplatin by inhibiting reducing capacity. Br J Cancer 126:1824-1833 (2022). PubMed: 35194192
- Yan K et al. Leonurine inhibits the TXNIP/NLRP3 and NF-κB pathways via Nrf2 activation to alleviate carrageenan-induced pleurisy in mice. Phytother Res 36:2161-2172 (2022). PubMed: 35285100
- Li J et al. CYT387, a JAK-Specific Inhibitor Impedes Osteoclast Activity and Oophorectomy-Induced Osteoporosis via Modulating RANKL and ROS Signaling Pathways. Front Pharmacol 13:829862 (2022). PubMed: 35345816
- Lee DS & Kim JE P2X7 Receptor Augments LPS-Induced Nitrosative Stress by Regulating Nrf2 and GSH Levels in the Mouse Hippocampus. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453462