リコンビナントRabMAb

Anti-GC1q R 抗体 [EPR23238-107] - BSA and Azide free (ab270038)

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Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
  • Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
  • Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
  • Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23238-107] to GC1q R - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free
    GC1q R 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23238-107] to GC1q R - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, A549, MCF7, WI-38 cell lysate; Rat, human and mouse spleen lysate; Human lung cancer lysate. IHC-P: Human, mouse and rat cerebral cortex. ICC/IF: HeLa and C2C12 cells. Flow: HeLa and C2C12 cells.

  • 特記事項

    ab270038 is the carrier-free version of ab270032. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
ICC/IF
Mouse
IHC-P
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Binds to the globular "heads" of C1Q thus inhibiting C1 activation.

  • 配列類似性

    Belongs to the MAM33 family.

  • 細胞内局在

    Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ASF/SF2 associated protein p32 antibody
    • C1q globular domain binding protein antibody
    • C1qBP antibody
    • C1QBP_HUMAN antibody
    • Complement component 1 q subcomponent binding protein antibody
    • Complement component 1 Q subcomponent binding protein mitochondrial antibody
    • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
    • GC1Q R antibody
    • GC1q R protein antibody
    • GC1q-R protein antibody
    • GC1QBP antibody
    • GC1QR antibody
    • globular domain of, C1q, receptor for antibody
    • Glycoprotein gC1qBP antibody
    • HABP 1 antibody
    • HABP1 antibody
    • Hyaluronan binding protein 1 antibody
    • Hyaluronan-binding protein 1 antibody
    • Mitochondrial matrix protein p32 antibody
    • p32 antibody
    • p32 splicing factor antibody
    • p33 antibody
    • Pre mrna splicing factor SF2 P32 subunit precursor antibody
    • SF2p32 antibody
    • Splicing factor SF2 associated protein antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GC1q R with ab270032 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in HeLa cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue). 

    Negative controls:
    -ve control 1: ab270032 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab150077 at 1/1000 dilution followed by ab33985 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex (PMID: 23924515). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GC1q R with ab270032 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling GC1q R with ab270032 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labeling GC1q R with ab270032 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in C2C12 cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue). 

    Negative controls:
    -ve control 1: ab270032 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab150077 at 1/1000 dilution followed by ab33985 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebral cortex (PMID: 9414106). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebral cortex (PMID: 9414106). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab270038 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab270038 は論文での使用が確認できていません。

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