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  1. Link

    gata3-antibody-epr16651-chip-grade-ab199428.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-GATA3 抗体 [EPR16651] - ChIP Grade (ab199428)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (11) Submit a question References (31)

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Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • ChIP - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • Immunocytochemistry/ Immunofluorescence - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • Flow Cytometry (Intracellular) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
  • Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16651] to GATA3 - ChIP Grade
  • Suitable for: Flow Cyt (Intra), ChIP, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 APC Carrier Free PE

こちらの製品もご検討ください

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二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-GATA3 antibody [EPR16651] - ChIP Grade
    GATA3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR16651] to GATA3 - ChIP Grade
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Flow Cyt (Intra), ChIP, WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: RAW 264.7 whole cell lysate (ab7187), SH-SY5Y cell extracts. EL4, HAP1 and Jurkat whole cell lysates. Mouse brain lysate. IHC-P: Human neuroblastoma and Human breast carcinoma tissue. ICC/IF: SH-SY5Y cells. Flow Cyt (intra): Jurkat cells. ChIP: Chromatin prepared from MCF7 cells.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR16651
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Developmental Biology
    • Lineage specification
    • Ectoderm

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-GATA3 antibody [EPR16651] (ab208895)
    • Alexa Fluor® 647 Anti-GATA3 antibody [EPR16651] (ab208896)
    • Alexa Fluor® 555 Anti-GATA3 antibody [EPR16651] (ab210672)
    • Anti-GATA3 antibody [EPR16651] - BSA and Azide free (ab214804)
    • APC Anti-GATA3 antibody [EPR16651] (ab225418)
    • PE Anti-GATA3 antibody [EPR16651] (ab225419)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant Human GATA3 protein (ab204202)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab199428の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.
ChIP
Use 2 µg for 25 µg of chromatin.
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P (7)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (2)
1/250.
特記事項
Flow Cyt (Intra)
1/500.
ChIP
Use 2 µg for 25 µg of chromatin.
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.

ターゲット情報

  • 機能

    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • 組織特異性

    T-cells and endothelial cells.
  • 関連疾患

    Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • 配列類似性

    Contains 2 GATA-type zinc fingers.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P23771 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2625 Human
    • Entrez Gene: 14462 Mouse
    • Omim: 131320 Human
    • SwissProt: P23771 Human
    • SwissProt: P23772 Mouse
    • Unigene: 524134 Human
    • Unigene: 313866 Mouse
    • 製品の状態

      There are 2 isoforms produced by alternative splicing.
    • 別名

      • GATA 3 antibody
      • GATA binding factor 3 antibody
      • GATA binding protein 3 antibody
      • GATA-binding factor 3 antibody
      • Gata3 antibody
      • GATA3_HUMAN antibody
      • HDR antibody
      • HDRS antibody
      • MGC2346 antibody
      • MGC5199 antibody
      • MGC5445 antibody
      • Trans acting T cell specific transcription factor GATA 3 antibody
      • Trans-acting T-cell-specific transcription factor GATA-3 antibody
      see all

    画像

    • Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      All lanes : Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : EL4 (mouse lymphoma T lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Exposure

      Lane1: 26 seconds

      Lane 2: 15 seconds

    • ChIP - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      ChIP - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

      Chromatin was prepared from MCF7 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab199428 (blue), and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the IgG control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach). Primers and probes are located in the first kb of the transcribed region.

    • Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      All lanes : Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : GATA3 knockout HAP1 whole cell lysate
      Lane 3 : Jurkat whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 48 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab199428 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab199428 was shown to recognize GATA3 in wild-type HAP1 cells as signal was lost at the expected MW in GATA3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GATA3 knockout samples were subjected to SDS-PAGE. Ab199428 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GATA3 with ab199428 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Immunocytochemistry/ Immunofluorescence - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y cells (Human neuroblastoma from bone marrow cells) labeling GATA3 with ab199428 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on SH-SY5Y cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:-
      -ve control 1 - ab199428 at 1/250 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry (Intracellular) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Flow Cytometry (Intracellular) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

      Intracellular Flow Cytometry analysis of Jurkat cells labelling GATA3 with ab199428 at 1/500 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluorr® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

      Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling GATA3 with ab199428 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human neuroblastoma tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Western blot - Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) at 1/1000 dilution + SH-SY5Y (Human neuroblastoma from bone marrow cells) cell extract at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
      Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

    プロトコール

    • ChIP protocols
    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    参考文献 (31)

    ab199428 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab199428 は 31 報の論文で使用されています。

    • Lin WH  et al. STAT3 phosphorylation at Ser727 and Tyr705 differentially regulates the EMT-MET switch and cancer metastasis. Oncogene 40:791-805 (2021). PubMed: 33262462
    • Guo G  et al. Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell 28:1040-1056.e6 (2021). PubMed: 33831366
    • Grassilli S  et al. Targeting the Vav1/miR-29b axis as a potential approach for treating selected molecular subtypes of triple-negative breast cancer. Oncol Rep 45:N/A (2021). PubMed: 33846812
    • Zhang Z  et al. GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression. Oncol Lett 21:231 (2021). PubMed: 33613720
    • Dong F  et al. Dihydroartemisinin inhibits the expression of von Willebrand factor by downregulation of transcription factor ERG in endothelial cells. Fundam Clin Pharmacol 35:321-330 (2021). PubMed: 33107067
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 11 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Embryonic stem cells)
    Permeabilization
    Yes - methanol
    Specification
    Embryonic stem cells
    Blocking step
    Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 21 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.5% Tween
    Specification
    Colon
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Tauseef Bashir Shah

    Verified customer

    投稿 Aug 13 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9 Tris EDTA
    Permeabilization
    No
    Specification
    Lymph node
    Blocking step
    Zytomed Systems Antibody Diluent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 11 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (B6 Kidney primary culture)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    B6 Kidney primary culture
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 20 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab94681 Tris EDTA pH9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 12 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab94681 Tris EDTA pH9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoids)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney H9 Organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab94681 Tris EDTA pH9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 23 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab94681 Tris EDTA pH9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Breast
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
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    Verified customer

    投稿 Dec 23 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA3 antibody [EPR16651] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab94681 Tris EDTA pH9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 23 2019

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