Anti-Gas1 抗体 (ab236618)
Key features and details
- Rabbit polyclonal to Gas1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Gas1 antibody
Gas1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Gas1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Gas1 aa 200 to the C-terminus.
Database link: P54826 -
ポジティブ・コントロール
- WB: SH-SY5Y and HEK-293 whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human pancreas and skin tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purity >95% -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236618の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/5000.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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特記事項 |
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WB
1/500 - 1/5000. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2619 Human
- Omim: 139185 Human
- SwissProt: P54826 Human
- Unigene: 65029 Human
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別名
- GAS-1 antibody
- Gas1 antibody
- GAS1_HUMAN antibody
see all
画像
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All lanes : Anti-Gas1 antibody (ab236618) at 1/500 dilution
Lane 1 : SH-SH5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution -
Paraffin-embedded human skin tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Gas1 (Green) using ab236618 at a 1/133 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
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Paraffin-embedded human pancreas tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab236618 は 3 報の論文で使用されています。
- Wang F et al. A positive feedback loop between TAZ and miR-942-3p modulates proliferation, angiogenesis, epithelial-mesenchymal transition process, glycometabolism and ROS homeostasis in human bladder cancer. J Exp Clin Cancer Res 40:44 (2021). PubMed: 33499877
- Wang X et al. Melatonin promotes bone marrow mesenchymal stem cell osteogenic differentiation and prevents osteoporosis development through modulating circ_0003865 that sponges miR-3653-3p. Stem Cell Res Ther 12:150 (2021). PubMed: 33632317
- Gao L et al. LncRNA LUADT1 Promotes Oral Squamous Cell Carcinoma Cell Proliferation by Regulating miR-34a/GAS1 Axis. Cancer Manag Res 12:3401-3407 (2020). PubMed: 32494204