Anti-GAPDH 抗体 [mAbcam 9484] - Loading Control (HRP) (ab9482)

製品の概要

  • 製品名
    Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (HRP)
  • 由来種
    Mouse
  • 標識
    HRP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Xenopus laevis, Chinese hamster
  • 免疫原

    Full length native protein from human erythrocytes.

  • 特記事項

    According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

    This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa). NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process.

ターゲット情報

画像

  • Lanes 1-4 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482) at 1/5000 dilution ((Blocked in 5% MILK))
    Lanes 5-8 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482) at 1/5000 dilution ((Blocked in 5% BSA))

    Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lanes 2 & 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lanes 3 & 7 : NIH 3T3 whole cell lysate (ab7179)
    Lanes 4 & 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40.2 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds

プロトコール

参考文献

This product has been referenced in:
  • Loganathan K  et al. Angiopoietin-1 deficiency increases renal capillary rarefaction and tubulointerstitial fibrosis in mice. PLoS One 13:e0189433 (2018). Read more (PubMed: 29293543) »
  • Ranieri A  et al. ß-Adrenergic regulation of cardiac type 2A protein phosphatase through phosphorylation of regulatory subunit B56d at S573. J Mol Cell Cardiol 115:20-31 (2018). Read more (PubMed: 29294329) »

See all 101 Publications for this product

レビューと Q&A

Many thanks for your valuable feedback on your recent experience of ab9482. In light of your feedback and that of several other customers we have removed all stock of this antibody and are offering our apologies for this unusual problem and a refun...

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Thank you for your phone enquiry earlier this morning. I have had a look at the records for this antibody (Ab9482) and we have experienced some mild batch variation. It has now been extensively tested in the laboratory again, giving good results but at...

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Thank you for your enquiry. The only difference between these two antibodies is that ab9482 is the HRP-conjugated version of ab9484. The advantage of ab9482 being HRP-conjugated is that there is no need for a secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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