製品の概要

  • 製品名

    Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)
    GAPDH 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (HRP)
  • 由来種

    Mouse
  • 標識

    HRP
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Xenopus laevis, Chinese hamster
  • 免疫原

    Full length native protein (purified) corresponding to Human GAPDH.

  • ポジティブ・コントロール

    • WB: HeLa, HEK-293, NIH/3T3 and PC-12 whole cell lysates.
  • 特記事項

    According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

    This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa).

NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process.

ターゲット情報

画像

  • All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482) at 1/1000 dilution

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : Hek293 Whole Cell Lysate
    Lane 3 : NIH 3T3 Whole Cel lLysate
    Lane 4 : PC12 Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40.2 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes

プロトコール

参考文献

This product has been referenced in:

  • Li R  et al. Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis. Mol Cancer 18:18 (2019). Read more (PubMed: 30704479) »
  • Wu YD  et al. Effects of storage culture media, temperature and duration on human adipose-derived stem cell viability for clinical use. Mol Med Rep 19:2189-2201 (2019). Read more (PubMed: 30664198) »
See all 128 Publications for this product

レビューと Q&A

1-10 of 23 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Several cell types, fibroblast, HEK293, LCL)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Several cell types, fibroblast, HEK293, LCL
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Apr 29 2019

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Mar 17 2016

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (12% polyacrylamide)
Sample
Human Cell lysate - whole cell (Huh-7.5 liver cells)
Specification
Huh-7.5 liver cells
Treatment
siRNA treated cells + untreated control lysate
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Sep 05 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Rat Tissue lysate - other (Cytosolic fractions from skeletal muscles)
Specification
Cytosolic fractions from skeletal muscles
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 31 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jan 22 2014

Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal muscle)
Loading amount
20 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. G.K Sakellariou

Verified customer

投稿 May 14 2013

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Non-reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Mar 30 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (Total Heart)
Loading amount
20 µg
Specification
Total Heart
Gel Running Conditions
Reduced Non-Denaturing (Native) (12% tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C

Abcam user community

Verified customer

投稿 Mar 29 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
30000 cells
Specification
293T
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Jun 10 2011

Application
Western blot
Sample
Human Cell lysate - other (HeLa cells)
Loading amount
500000 cells
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Nov 12 2010

1-10 of 23 Abreviews or Q&A

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