Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (liver, HepG2)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
liver, HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: GOAT ANTI-MOUSE SECONDARY ANTIBODY
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
12 second(s)
Bands
Specific: 37 kDa

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投稿 Dec 04 2017

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