Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Inconclusive
Abreviews
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Liver, RPE, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Liver, RPE, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/5000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Detection

Detection method
Licor Odyssey Clx
Exposure
15 minute(s) and 0 second(s)
Positive control
HEK cell lysate, mouse liver lysate

Additional data

Additional Notes
I was previously told to mark inconclusive in a blot for an application that is not guaranteed or purported (e.g. for zebrafish) if this is not the case, change to 1. Lane 1: HEK cells Lane 2: hfRPE cells Lane 3: hfRetina Lane 4: Mouse liver Lane 5: Mouse RPE Lane 6: Mouse retina Lane 7: Zebrafish liver Lane 8: Zebrafish retina PVDF membrane RIPA lysis buffer with mixed phosphatase and protease inhibitor cocktail
Abcam response
The antibody is guaranteed to work with Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Cynomolgus monkey, Chinese hamster species. As lanes 1 and 2 indicate - human works and lanes 5 and 6 show - mouse works. The zebrafish sample which customer was testing, unfortunately did not work.

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投稿 Mar 24 2017

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