Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (PERIPHERAL BLOOD MONONUCLEAR CELLS)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
30 µg
Specification
PERIPHERAL BLOOD MONONUCLEAR CELLS
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% NFM
Dilution
1/750

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: DAKO ANTI MOUSE
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 37 kDa

Mr. Siddharth Mehra

Verified customer

投稿 Jun 02 2016

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