Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-mouse secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 37 kDa

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投稿 Mar 14 2016

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