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  1. Link

    gapdh-antibody-mabcam-9484-loading-control-ab9484.pdf

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Anti-GAPDH 抗体 [mAbcam 9484] - Loading Control (ab9484)

  • Datasheet
  • SDS
Reviews (56)Q&A (7)References (742)

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Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

Key features and details

  • Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 790 HRP

こちらの製品もご検討ください

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Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay Kit (Colorimetric) (ab204732)

関連製品

製品の概要

  • 製品名

    Anti-GAPDH antibody [mAbcam 9484] - Loading Control
    GAPDH 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
  • 由来種

    Mouse
  • 特異性

    In western blot, this product typically gives a lower signal in rat lysates compared to human and mouse lysates.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster
    交差が予測される動物種: Rabbit, Dog, Cynomolgus monkey
  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: A431, Jurkat, HEK293, HeLa, NIH 3T3, PC-12, EBTr and CHO cell lysates; Rat brain, chicken and pig liver and Xenopus laevis embryo tissue lysates. IHC-P: Human liver tissue.
  • 特記事項

    For Western blotting, do not use milk for blocking. Our labs have extensively tested the blocking conditions for this antibody and recommend using 5% BSA for 1 hour. The comparison data is shown in the images section. 

    This antibody clone [mAbcam 9484] is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 精製度

    IgG fraction
  • ポリ/モノ

    モノクローナル
  • クローン名

    mAbcam 9484
  • ミエローマ

    Sp2/0-Ag14
  • アイソタイプ

    IgG2b
  • 軽鎖の種類

    kappa
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Alternative Versions

    • Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)
    • Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095)
    • Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184578)
    • Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)
    • HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9484の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (53)
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 36 kDa.

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 36 kDa.

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • パスウェイ

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 配列類似性

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻訳後修飾

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 細胞内局在

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 374193 Chicken
    • Entrez Gene: 281181 Cow
    • Entrez Gene: 403755 Dog
    • Entrez Gene: 2597 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Entrez Gene: 396823 Pig
    • Entrez Gene: 100009074 Rabbit
    • Entrez Gene: 24383 Rat
    • Entrez Gene: 685186 Rat
    • Entrez Gene: 380259 Xenopus laevis
    • Omim: 138400 Human
    • SwissProt: P00356 Chicken
    • SwissProt: P10096 Cow
    • SwissProt: Q28259 Dog
    • SwissProt: P04406 Human
    • SwissProt: P16858 Mouse
    • SwissProt: P00355 Pig
    • SwissProt: P46406 Rabbit
    • SwissProt: P04797 Rat
    • SwissProt: P51469 Xenopus laevis
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 475698 Mouse
    • Unigene: 129558 Rat
    • Unigene: 91450 Rat
    • Unigene: 995 Xenopus laevis
    see all
  • 別名

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

画像

  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : NIH 3T3 Whole Cell Lysate
    Lane 3 : PC12 Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab9484 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    IHC image of GAPDH staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9484, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/5000 dilution

    Lane 1 : Hela whole cell (Human)
    Lane 2 : 3T3 cell (Mouse)
    Lane 3 : Rat brain
    Lane 4 : Xenopus laevis embryo
    Lane 5 : Chicken Liver
    Lane 6 : EBTr cell (Cow)
    Lane 7 : CHO cell (Chinese hamster)
    Lane 8 : Pig liver

    Secondary
    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    The membrane was blocked in 5% BSA in TBST for 1 hour, then incubated for 1 hour in primary antibody diluted in TBST.

  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Lanes 1-5 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution (Blocked in 5% milk)
    Lanes 6-10 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution (Blocked in 5% BSA)

    Lanes 1 & 6 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lanes 2 & 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lanes 3 & 8 : A-431 whole cell lysate (ab7909)
    Lanes 4 & 9 : Jurkat whole cell lysate (ab7899)
    Lanes 5 & 10 : HEK-293 whole cell lysate (ab7902)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-5 : Goat anti-Mouse (HRP conjugated) at 1/5000 dilution
    Lanes 6-10 : Goat anti-Mouse (HRP conjugated) at 1/5000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    The membrane 1-5 was blocked in 5% milk (1 hour). The membrane 6-10 was blocked in 5% BSA (1 hour). Milk is not a suitable blocking agent and significantly decreases the signal on the membrane.

  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 0.5 µg/ml + HeLa cell lysate

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 36 kDa


    Exposure time: 30 seconds

プロトコール

  • Mouse on Mouse staining protocol

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (742)

ab9484 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9484 は 742 報の論文で使用されています。

  • Wu Y  et al. IL-17 predicts the effect of TACE combined with apatinib in hepatocellular carcinoma. Clin Hemorheol Microcirc 77:37-47 (2021). PubMed: 32651310
  • Wesolowski R  et al. Myeloid transformation by MLL-ENL depends strictly on C/EBP. Life Sci Alliance 4:N/A (2021). PubMed: 33144337
  • Jin H  et al. Oncogenic role of MIR516A in human bladder cancer was mediated by its attenuating PHLPP2 expression and BECN1-dependent autophagy. Autophagy 17:840-854 (2021). PubMed: 32116109
  • Jiang X & Chen D Circular RNA hsa_circ_0000658 inhibits osteosarcoma cell proliferation and migration via the miR-1227/IRF2 axis. J Cell Mol Med 25:510-520 (2021). PubMed: 33264494
  • Gao S  et al. LncRNA AK148321 alleviates neuroinflammation in LPS-stimulated BV2 microglial cell through regulating microRNA-1199-5p/HSPA5 axis. Life Sci 266:118863 (2021). PubMed: 33301806
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 63 Abreviews or Q&A

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Kyoto and MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
HeLa Kyoto and MDA-MB-231
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Christos Efstathiou

Verified customer

投稿 Feb 24 2022

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (liver, HepG2)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
liver, HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 04 2017

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 01 2017

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Liver, RPE, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Liver, RPE, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 24 2017

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
HEK cells
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 17 2017

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Liver
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 14 2016

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (muscle)
Gel Running Conditions
Reduced Denaturing (9)
Loading amount
50 µg
Specification
muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Baptiste Jude

Verified customer

投稿 Oct 07 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H1299 Lung cancer cell line)
Permeabilization
Yes - 0.1% v/v TritonX-100
Specification
H1299 Lung cancer cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

投稿 Jun 06 2016

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (PERIPHERAL BLOOD MONONUCLEAR CELLS)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
30 µg
Specification
PERIPHERAL BLOOD MONONUCLEAR CELLS
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Siddharth Mehra

Verified customer

投稿 Jun 02 2016

Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 17 2016

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