Anti-GAPDH 抗体 [mAbcam 9484] - BSA and Azide free (ab174646)
製品の概要
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製品名Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free
GAPDH 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [mAbcam 9484] to GAPDH - BSA and Azide free
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由来種Mouse
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アプリケーション適用あり: Flow Cyt, IHC-P, WBmore details
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種交差性交差種: Mouse, Rat, Rabbit, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster
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免疫原
Full length native protein (purified) corresponding to Human GAPDH.
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特記事項
This antibody clone is manufactured by Abcam.
This product is the PBS-only buffer version of ab9484.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.4
Constituent: PBS -
Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名mAbcam 9484
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ミエローマSp2/0-Ag14
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アイソタイプIgG2b
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軽鎖の種類kappa
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研究分野
関連製品
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Alternative Versions
- Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 680) (ab184095)
- Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 790) (ab184578)
- Anti-GAPDH antibody [mAbcam 9484] (Alexa Fluor® 647) (ab196697)
- Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482)
- Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab174646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40.2 kDa. NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody following concerning customer feedback on the lack of signal with some of the vials. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images). This change in the characteristics of the antibody is due to a recent update to the production process. |
ターゲット情報
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機能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
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配列類似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
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翻訳後修飾S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
細胞内局在Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
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参照データベース
- Entrez Gene: 374193 Chicken
- Entrez Gene: 281181 Cow
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 396823 Pig
- Entrez Gene: 100009074 Rabbit
- Entrez Gene: 24383 Rat
see all -
別名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)](https://www.abcam.co.jp/ps/products/174/ab174646/Images/ab174646-284222-AB9484ap521976.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)This IHC data was generated using the same antibody clone in a different buffer formulation (ab9484).
IHC image of GAPDH staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9484, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
データシートおよび資料
参考文献
ab174646 has not yet been referenced specifically in any publications.
