製品の概要

  • 製品名

    Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free
    GAPDH 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [mAbcam 9484] to GAPDH - BSA and Azide free
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, IHC-P, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Rabbit, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster
  • 免疫原

    Full length native protein (purified) corresponding to Human GAPDH.

  • 特記事項

    This antibody clone is manufactured by Abcam.

    This product is the PBS-only buffer version of ab9484.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab174646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40.2 kDa.

NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody following concerning customer feedback on the lack of signal with some of the vials. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images). This change in the characteristics of the antibody is due to a recent update to the production process.

ターゲット情報

画像

  • This IHC data was generated using the same antibody clone in a different buffer formulation (ab9484). 

    IHC image of GAPDH staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9484, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

参考文献

ab174646 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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