Application
Western blot
Sample
Human Cell lysate - whole cell (H1944 lung adenocarcinoma)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
18 µg
Specification
H1944 lung adenocarcinoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS-TWEEN
Dilution
1/3000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Donkey anti raddbit antibody
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Li-COR oydssey scanner
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Additional data
Additional Notes
gave clean crisp band in 4 different samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Wei-Ting Lu
Verified customer
投稿 Aug 20 2018