Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7)
Loading amount
20 µg
Specification
huh-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
western lightning chemiluminescent reage
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 40 kDa
Additional data
Additional Notes
Lane 1 was loaded with 2ug, lane 2 with 20ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 01 2009