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  1. Link

    gapdh-antibody-loading-control-ab9483.pdf

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Anti-GAPDH 抗体 - Loading Control (ab9483)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (129)

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Western blot - Anti-GAPDH antibody - Loading Control (ab9483)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)

Key features and details

  • Goat polyclonal to GAPDH - Loading Control
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-GAPDH antibody - Loading Control
    GAPDH 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to GAPDH - Loading Control
  • 由来種

    Goat
  • アプリケーション

    適用あり: WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human GAPDH.

  • ポジティブ・コントロール

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3. This antibody also gave a positive signal in Human brain tissue lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
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    • Metabolic signaling pathways
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    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)
  • Related Products

    • Anti-SDHB antibody [21A11AE7] (ab14714)
    • Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
    • Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
    • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9483の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (5)
1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 35.8 kDa).
ICC/IF (1)
Use a concentration of 1 µg/ml.
特記事項
WB
1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 35.8 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • パスウェイ

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 配列類似性

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻訳後修飾

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 細胞内局在

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2597 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Omim: 138400 Human
    • SwissProt: P04406 Human
    • SwissProt: P16858 Mouse
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 475698 Mouse
    see all
  • 別名

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

画像

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9483)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9483)
    All lanes : Anti-GAPDH antibody - Loading Control (ab9483) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 35.8 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
    ICC/IF image of ab9483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9483, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 1µg/ml.
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)This image is courtesy of an Abreview submitted by Kirk McManus

    ab9483 staining GAPDH in HeLa by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehydeand. Samples were incubated with primary antibody (1/200) for 30 minutes. An undiluted Alexa Fluor® 568-conjugated Donkey anti-goat polyclonal was used as the secondary antibody.

    See Abreview

プロトコール

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (129)

ab9483 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9483 は 129 報の論文で使用されています。

  • Cave DD  et al. Nodal-induced L1CAM/CXCR4 subpopulation sustains tumor growth and metastasis in colorectal cancer derived organoids. Theranostics 11:5686-5699 (2021). PubMed: 33897875
  • Chen FX  et al. Intramuscular accumulation of pentadecanoic acid activates AKT1 to phosphorylate NCOR1 and triggers FOXM1-mediated apoptosis in the pathogenesis of sarcopenia. Am J Transl Res 12:5064-5079 (2020). PubMed: 33042406
  • Chen FX  et al. Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting BAK1. Int J Biol Sci 16:1481-1494 (2020). PubMed: 32226296
  • Alanazi AM  et al. Liposomal Resveratrol and/or Carvedilol Attenuate Doxorubicin-Induced Cardiotoxicity by Modulating Inflammation, Oxidative Stress and S100A1 in Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32079097
  • Wing TT  et al. OPN binds alpha V integrin to promote endothelial progenitor cell incorporation into vasculature. Reproduction 159:465-478 (2020). PubMed: 31990676
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-GAPDH antibody - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (RPE cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
RPE cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Nov 04 2019

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification
U2OS osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Feb 21 2014

Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (skeletal muscle)
Loading amount
50 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Sep 25 2012

Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (aorta)
Loading amount
20 µg
Specification
aorta
Gel Running Conditions
Reduced Denaturing (Bis-tris 4-12%)
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 May 20 2010

Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Blocking step
normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. Natalie Farny

Verified customer

投稿 Oct 23 2009

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GAPDH antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Read More

DR. Sophie Pezet

Verified customer

投稿 Jul 31 2009

Immunocytochemistry/ Immunofluorescence abreview for Anti-GAPDH antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Read More

DR. Kirk Mcmanus

Verified customer

投稿 Oct 03 2006

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