Application
Western blot
Sample
Ferret Tissue lysate - whole (Ferret whole brain tissue)
Gel Running Conditions
Reduced Denaturing (3-8% Tris-acetate gel, NuPage)
Loading amount
40 µg
Specification
Ferret whole brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5% milk in TBS-T)
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: HRP Goat anti Mouse, Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL-Perkin Elmer
Exposure
10 second(s)
Bands
Specific: 37 kDa
Positive control
Same band size (37kDa) obtained with mouse whole brain tissue samples in different experiments.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Francis Djankpa
Verified customer
投稿 Jul 11 2016