Name: Anti-GAP43 antibody - Neuronal Marker
SKU: ab16053
Rating: 4 (12 reviews)

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Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)

Key features and details

Rabbit polyclonal to GAP43 - Neuronal Marker
Suitable for: WB, IHC (PFA fixed)
Reacts with: Mouse, Rat
Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Anti-GAP43 antibody [EP890Y] (ab75810)

異なるロット間での安定した再現性
容易なスケールアップ
評価試験による特異性の確認済み
倫理基準に準拠 - アニマル・フリーの生産

製品名

Anti-GAP43 antibody - Neuronal Marker
GAP43 一次抗体製品一覧
製品の詳細

Rabbit polyclonal to GAP43 - Neuronal Marker
由来種

Rabbit
特異性

This antibody does not perform well in IHC-P (abreview 21090, 21089)
アプリケーション

適用あり: WB, IHC (PFA fixed)more details
適用なし: IHC-P
種交差性

交差種: Mouse, Rat
交差が予測される動物種: Human, Chimpanzee
免疫原

Synthetic peptide corresponding to Rat GAP43 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab16379)
特記事項

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度

Immunogen affinity purified
ポリ/モノ

ポリクローナル
アイソタイプ

IgG
研究分野
- Neuroscience
- Development

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Recombinant Protein
- Recombinant Human GAP43 protein (ab114188)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab16053の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(3)	Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa).
IHC (PFA fixed)		Use at an assay dependent concentration.

特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa).
IHC (PFA fixed) Use at an assay dependent concentration.

追加情報

Is unsuitable for IHC-P.

機能

This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
配列類似性

Belongs to the neuromodulin family.
Contains 1 IQ domain.
翻訳後修飾

Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
細胞内局在

Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
Target information above from: UniProt accession P17677 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 460599 Chimpanzee
- Entrez Gene: 2596 Human
- Entrez Gene: 14432 Mouse
- Entrez Gene: 29423 Rat
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- SwissProt: P06837 Mouse
- SwissProt: P07936 Rat
- Unigene: 134974 Human
- Unigene: 1222 Mouse
- Unigene: 10928 Rat
see all
別名
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
- Calmodulin binding protein P 57 antibody
- F1 antibody
- GAP 43 antibody
- GAP43 antibody
- Growth Associated Protein 43 antibody
- Growth-associated protein 43 antibody
- Nerve Growth Related Peptide antibody
- Nerve growth related peptide GAP43 antibody
- NEUM_HUMAN antibody
- Neural phosphoprotein B 50 antibody
- Neural phosphoprotein B-50 antibody
- Neuromodulin antibody
- Neuron growth associated protein 43 antibody
- PP46 antibody
- Protein F1 antibody
- QtrA-11580 antibody
- QtrA-13071 antibody
see all

Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)

All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml

Lane 1 : spinal cord chung rat 1
Lane 2 : spinal cord chung rat 2
Lane 3 : spinal cord chung rat 3

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution

Performed under reducing conditions.

Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa (possible multimer)
Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom

Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)

Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 8 minutes

Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab16053 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab16053 は 55 報の論文で使用されています。

Shi W et al. Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro. Amino Acids 53:11-22 (2021). PubMed: 33245424
Toro CA et al. The Human ApoE4 Variant Reduces Functional Recovery and Neuronal Sprouting After Incomplete Spinal Cord Injury in Male Mice. Front Cell Neurosci 15:626192 (2021). PubMed: 33679326
Liu H et al. SARM1 promotes neuroinflammation and inhibits neural regeneration after spinal cord injury through NF-?B signaling. Theranostics 11:4187-4206 (2021). PubMed: 33754056
Jeanette H et al. YAP and TAZ regulate Schwann cell proliferation and differentiation during peripheral nerve regeneration. Glia 69:1061-1074 (2021). PubMed: 33336855
Ribas VT et al. AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo. Cell Death Dis 12:213 (2021). PubMed: 33637688

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 15 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (spinal cord)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris)

Loading amount

10 µg

Specification

spinal cord

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C

Abcam user community

Verified customer

投稿 May 17 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (mouse brain (hippocampus))

Specification

mouse brain (hippocampus)

Fixative

4% PFA fixed

Antigen retrieval step

None

Permeabilization

Yes - 0,1% Saponin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Nov 19 2012

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (mouse lung (bronchus))

Specification

mouse lung (bronchus)

Fixative

Methanol

Permeabilization

Yes - 0,1% Saponin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Nov 12 2012

Application

Western blot

Sample

Mouse Tissue lysate - whole (brain tissue)

Loading amount

10 µg

Specification

brain tissue

Gel Running Conditions

Reduced Non-Denaturing (Native) (5-16% gel)

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Nov 09 2012

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (dorsal root ganglia)

Specification

dorsal root ganglia

Fixative

Methanol

Permeabilization

Yes - 0,1% Saponin / PBS

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Dec 12 2011

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (in situ spinal cord)

Specification

in situ spinal cord

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid pH6

Permeabilization

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

MR. Carl Hobbs

Verified customer

投稿 Nov 02 2010

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Head)

Specification

Head

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid pH6

Permeabilization

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

MR. Carl Hobbs

Verified customer

投稿 Nov 02 2010

Question

We are planing to use anti-GAP43 to label regenerating axons of the sciatic nerves in mice (immunofluorescence). Ab7462 is known to work well, but it is either discontinuous or change to another Catalog number (?). We sincerely hope you can find a replacement for us.

Abcam community

Verified customer

Asked on Feb 04 2013

Answer

We would recommend using ab16053 in place of the discontinued item. Ab16053 has been tested in and is guaranteed in IF on mouse samples. This product has also been featured in 5 publications which we are aware of and has excellent Abreviews. More information about this may be found at: https://www.abcam.com/gap43-antibody-neuronal-marker-ab16053.html Abcam also carries a number of reagents for IF staining, including serums and blocking reagents, mounting media and background reducing buffers. Our BrightMount Plus Aqueous Mounting Medium for Fluorescent Staining (ab103748; https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html) is specifically designed for use in IF and would protect against photo-bleaching of your samples.

Abcam Scientific Support

Answered on Feb 04 2013

Question

The following is the answer to your questionnaire.

1) Abcam product code ab16053

2) Abcam order reference number or product batch number

Lot: GR42948-1

3) Description of the problem

I perform the immunofluorescent stain on my cultured hippocampal neuron with this antibody, no matter fixed with 4%PFA or pure methanol, I can not get the specific stain as the product sheet showed

4) Sample preparation:

Species: mouse

Type of sample: primary cultured hippocampal neuron from mouse E18 embryonic

Sample preparation

The cultured hippocampal neuron was washed twice with 1XPBS, and then fixed with 4%PFA 14min or fixed with pre-cooled pure methanol 10min. Aften then cells were penetrated with 0.2% triton-X100 in 1XPBS for 8 min and blocked in 1% BSA in 1XPBS for 1 hour. Next cells were incubate with diluted antibody solution(1:500, as the product sheet say) at 4 degree overnight. The second day the cells were wash with 1XPBS for four times 5min each time. Then incubate with secondary antibody(Alexa 633 or Alexa 555 from Invitrogen 1:2000) for 1 hours at room temperature. After wash, finally the coverslip were mounted with prolong mounting solution(Invitrogen). The picture was taken with OMX superesolution microscope or zeiss fluorescent microscope.

Positive control: phalloindin 488 stain and or belta tubulin antibody stain.

Negative control

Abcam community

Verified customer

Asked on Mar 01 2012

Answer

Thank you for your reply.

Your protocol looks good. The one recommendation I could make would be to increase the dilution of the primary and secondary (especially) to see if that improves the staining.
You can try the primary at 1:250 and the secondary at 1:200 and see if that helps.

If this does not improve the staining, we would be happy to replace or refund the product. Please let me know if this does not help and what you would prefer as compensation.

Abcam Scientific Support

Answered on Mar 01 2012

Question

Phone call reporting high background with ab166053 when used for immunohistochemical staining of rat brain.

Abcam community

Verified customer

Asked on Jan 31 2012

Answer

Thank you for calling us and for alerting us to the problem you are experiencing with anti-GAP43 antibody (ab16053). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, Ihave attachedour questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary.

If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Abcam Scientific Support

Answered on Jan 31 2012

1-10 of 15 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (55)

レビューと Q&A

Western blot abreview for Anti-GAP43 antibody - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker

Immunohistochemistry (Frozen sections) abreview for Anti-GAP43 antibody - Neuronal Marker

Western blot abreview for Anti-GAP43 antibody - Neuronal Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-GAP43 antibody - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker