Anti-GAP43 抗体 - Neuronal Marker (ab16053)
Key features and details
- Rabbit polyclonal to GAP43 - Neuronal Marker
- Suitable for: WB, IHC (PFA fixed)
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GAP43 antibody - Neuronal Marker
GAP43 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GAP43 - Neuronal Marker -
由来種
Rabbit -
特異性
This antibody does not perform well in IHC-P (abreview 21090, 21089) -
アプリケーション
適用あり: WB, IHC (PFA fixed)more details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human, Chimpanzee -
免疫原
Synthetic peptide corresponding to Rat GAP43 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16379) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16053の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa).
|
IHC (PFA fixed) |
Use at an assay dependent concentration.
|
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa). |
IHC (PFA fixed)
Use at an assay dependent concentration. |
ターゲット情報
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機能
This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. -
配列類似性
Belongs to the neuromodulin family.
Contains 1 IQ domain. -
翻訳後修飾
Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. -
細胞内局在
Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 460599 Chimpanzee
- Entrez Gene: 2596 Human
- Entrez Gene: 14432 Mouse
- Entrez Gene: 29423 Rat
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- SwissProt: P06837 Mouse
see all -
別名
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
see all
画像
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All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml
Lane 1 : spinal cord chung rat 1
Lane 2 : spinal cord chung rat 2
Lane 3 : spinal cord chung rat 3
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa (possible multimer) -
Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
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Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (65)
ab16053 は 65 報の論文で使用されています。
- Iwasa K et al. GPR137 Inhibits Cell Proliferation and Promotes Neuronal Differentiation in the Neuro2a Cells. Neurochem Res 48:996-1008 (2023). PubMed: 36436172
- Wu Q et al. YAP signaling in horizontal basal cells promotes the regeneration of olfactory epithelium after injury. Stem Cell Reports 17:664-677 (2022). PubMed: 35148842
- Wang Q et al. Flavonoid Extract from Propolis Provides Cardioprotection following Myocardial Infarction by Activating PPAR-γ. Evid Based Complement Alternat Med 2022:1333545 (2022). PubMed: 35928246
- Jin L et al. Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling. Cell Death Dis 13:759 (2022). PubMed: 36055989
- Liu J et al. Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury. Neural Regen Res 17:673-681 (2022). PubMed: 34380909