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Anti-Ganglioside GD3 抗体 [R24] (ab11779)

Submit a review Q&A (2)References (20)
Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (ab11779)
  • Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)

Key features and details

  • Mouse monoclonal [R24] to Ganglioside GD3
  • Suitable for: IHC-P, ICC/IF, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG3

製品の概要

  • 製品名

    Anti-Ganglioside GD3 antibody [R24]

  • 製品の詳細

    Mouse monoclonal [R24] to Ganglioside GD3

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, ICC/IF, IHC-Frmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human Ganglioside GD3. This antibody was raised against SK-MEL-28 melanoma cell line.

  • ポジティブ･コントロール

    • ICC/IF: SH-SY5Y cells. IHC-P: Human melanoma tissue. IHC-Fr: Human melanoma tisue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Affinity purified from tissue culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    R24

  • アイソタイプ

    IgG3

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11779の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-Fr
1/40.
特記事項
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-Fr
1/40.

ターゲット情報

  • 関連性

    Almost all melanomas, astrocytomas, a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen. It is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in Humans via enhancement of cytotoxic and proliferative response of lymphocytes.

  • 別名

    • GD3 antibody

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    ICC/IF image of ab11779 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11779, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)

    Frozen section of human melanoma tissue stained for Ganglioside GD3 with ab11779 at 1/40 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)
    IHC image of ab11779 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11779, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (20)

ab11779 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11779 は 20 報の論文で使用されています。

  • Ramos RI  et al. Upregulation of cell surface GD3 ganglioside phenotype is associated with human melanoma brain metastasis. Mol Oncol 14:1760-1778 (2020). PubMed: 32358995
  • Rosenstock P  et al. Sialylation of Human Natural Killer (NK) Cells is Regulated by IL-2. J Clin Med 9:N/A (2020). PubMed: 32545211
  • Das A  et al. Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus. Nat Microbiol 5:1069-1078 (2020). PubMed: 32451473
  • Tong W  et al. Vaccination with Tumor-Ganglioside Glycomimetics Activates a Selective Immunity that Affords Cancer Therapy. Cell Chem Biol 26:1013-1026.e4 (2019). PubMed: 31105061
  • Deng Q  et al. Tissue-specific microRNA expression alters cancer susceptibility conferred by a TP53 noncoding variant. Nat Commun 10:5061 (2019). PubMed: 31699989
View all Publications for this product

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Dear abcam technical support team: This customer would like to order ab11779 (Anti-Ganglioside GD3 antibody [R24]), she would likes to order this antibody and test in ICC assay with mouse sample, would you please help this customer to create a discount code, the information of this customer as follow:

Could you please help this customer to solve the problem? Thanks for your kindly help.
Best regards

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Answer

I am pleased to hear the customer would like to make use of our testing discount offer and test ab11779 in mouse. I have arranged the following code:

DISCOUNT CODE: #####
Expiration date: ######

This code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscounthttps://www.abcam.com/collaborationdiscount.

Any feedback that the customer can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wish the customer luck with their research.

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Question

What is the concentration of this antibody?

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Answer

Thank you for your enquiry. The concentration of this antibody is 400 ug/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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