Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6925] to GAMT
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
製品名Anti-GAMT antibody [EPR6925]
GAMT 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6925] to GAMT
アプリケーション適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP
種交差性交差種: Rat, Human
Synthetic peptide within Human GAMT aa 150-250. The exact sequence is proprietary.
- Human fetal liver, HepG2 and SH-SY5Y lysates; Human liver tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解離定数（KD 値）KD = 7.40 x 10 -11 M Learn more about KD
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab126736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
組織特異性Expressed in liver.
パスウェイAmine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 2/2.
関連疾患Defects in GAMT are the cause of guanidinoacetate methyltransferase deficiency (GAMT deficiency) [MIM:612736]. GAMT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, intractable seizures and movement disturbances, severe depletion of creatine/phosphocreatine in the brain, and accumulation of guanidinoacetic acid (GAA) in brain and body fluids.
配列類似性Belongs to the RMT2 methyltransferase family.
- Information by UniProt
- CCDS2 antibody
- Epididymis secretory protein Li 20 antibody
- GAMT antibody
ab126736 at 1/100 dilution, staining GAMT in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-GAMT antibody [EPR6925] (ab126736) at 1/1000 dilution
Lane 1 : Human fetal liver Lysate
Lane 2 : HepG2 lysate
Lane 3 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
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