Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-gamma Tubulin 抗体 [GTU-88] - Centrosome Marker (ab11316)

Reviews (17)Q&A (4)References (150)
Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
  • Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
  • Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

Key features and details

  • Mouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Chicken, Cow, Dog, Human, African green monkey, Chinese hamster
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker
    gamma Tubulin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC, WBmore details

  • 種交差性

    交差種: Mouse, Chicken, Cow, Dog, Human, African green monkey, Chinese hamster

  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11316の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use a concentration of 1 - 2 µg/ml.
WB (6)
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
特記事項
ICC
Use a concentration of 1 - 2 µg/ml.
WB
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

画像

  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    All lanes : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution

    Lane 1 : HeLa cell line lysates
    Lane 2 : U87 cell line lysates
    Lane 3 : COS7 cell line lysates
    Lane 4 : P19 cell line lysates
    Lane 5 : Hepa1-6 cell line lysates
    Lane 6 : CHO cell line lysates
    Lane 7 : MDBK cell line lysates
    Lane 8 : MDCK cell line lysates

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 48 kDa

  • Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.

    This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.

    See Abreview

  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Lane 1 : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution
    Lane 2 : Negative control (secondary antibody only)

    All lanes : Whole cell extract of chicken fibroblasts

    Secondary
    All lanes : Alkaline Phosphatase conjugated goat anti-mouse IgG

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.

参考文献 (150)

ab11316 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11316 は 150 報の論文で使用されています。

  • Li C  et al. SRPS associated protein WDR60 regulates the multipolar-to-bipolar transition of migrating neurons during cortical development. Cell Death Dis 12:75 (2021). PubMed: 33436552
  • Ryu H  et al. The molecular dynamics of subdistal appendages in multi-ciliated cells. Nat Commun 12:612 (2021). PubMed: 33504787
  • Charruyer A  et al. Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis. Aging Cell 20:e13310 (2021). PubMed: 33524216
  • Park HS  et al. ARP-T1-associated Bazex-Dupré-Christol syndrome is an inherited basal cell cancer with ciliary defects characteristic of ciliopathies. Commun Biol 4:544 (2021). PubMed: 33972689
  • Schilpp C  et al. TGF-ß1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei. Pflugers Arch 473:287-311 (2021). PubMed: 33386991
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 21 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (Human Foreskin Fibroblasts (HFF))
Permeabilization
Yes - Triton X-100
Specification
Human Foreskin Fibroblasts (HFF)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 15 2020

Western blot abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 ng/ml LPS
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Nov 13 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT29)
Permeabilization
Yes - 0.2% TritonX100 in PBS
Specification
HT29
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 07 2012

Western blot abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Nov 07 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
No
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Methanol
Read More

Abcam user community

Verified customer

投稿 Jun 28 2012

Western blot abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jun 01 2012

Immunohistochemistry free floating abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain, ependymal cells lining the ventricular wall)
Specification
Brain, ependymal cells lining the ventricular wall
Read More

Mr. Karthikeyan Devaraju

Verified customer

投稿 Jun 07 2011

Western blot abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Specification
MRC5VA Lung Epithelial Fibroblasts
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Apr 13 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Liver cancer cell line)
Permeabilization
Yes - 0.5% Triton
Specification
Liver cancer cell line
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 31 2011

Question

Dear Abcam, I am looking for a gamma-tubulin antibody that will work in zebrafish. I will mostly be using this antibody for western blots. In this regard, I am interested in your product- ab11316 [Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker]. Your data sheet lists a number of species, but zebrafish is not one of them. Does it mean that it does not recognise zebrafish gamma-tubulin, or just that it has not yet been tested? If it has not been tested, can I avail of your "100% Testing Discount" offer to test this antibody in zebrafish for westerns? Thank you very much for your time

Read More
Answer

DISCOUNT CODE: XXXXXX-XXXXX Expiration date: DD MM 20YY Thank you very much for your interest in ab11316. To our knowledge, ab11316 has not been tested in Zebrafish. I am very pleased to hear you would like to accept our offer and test ab11316 in zebrafish. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for zebrafish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. For your information, the list of Abcam antibodies tested in zebrafish can be found via the following link : www.abcam.com/zebrafish

Read More

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com