Anti-gamma Synuclein/SNCG 抗体 [EP1539Y] (ab52633)
![Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)](https://www.abcam.co.jp/ps/products/52/ab52633/Images/ab52633-393613-anti-gamma-synucleinsncg-antibody-ep1539y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1539Y] to gamma Synuclein/SNCG
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-gamma Synuclein/SNCG antibody [EP1539Y]
gamma Synuclein/SNCG 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1539Y] to gamma Synuclein/SNCG -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide within gamma Synuclein/SNCG aa 100-200. The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: HeLa and SK-BR-3 cell lysates; Human fetal brain lysate. IHC-P: Human brain.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1539Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52633の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. -
組織特異性
Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. -
配列類似性
Belongs to the synuclein family. -
翻訳後修飾
Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. -
細胞内局在
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. - Information by UniProt
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参照データベース
- Entrez Gene: 6623 Human
- Entrez Gene: 64347 Rat
- Omim: 602998 Human
- SwissProt: O76070 Human
- SwissProt: Q63544 Rat
- Unigene: 349470 Human
- Unigene: 2883 Rat
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別名
- BCSG1 antibody
- Breast cancer specific gene 1 protein antibody
- Breast cancer specific protein 1 antibody
see all
画像
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Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)All lanes : Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNCG knockout HeLa cell lysate
Lane 3 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 13 kDaLanes 1-3: Merged signal (red and green). Green - ab52633 observed at 16 kDa. Red - loading control ab8245 observed at 36 kDa.
ab52633 Anti-gamma Synuclein/SNCG antibody [EP1539Y] was shown to specifically react with gamma Synuclein/SNCG antibody in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265623 (knockout cell lysate ab257694) was used. Wild-type and gamma Synuclein/SNCG knockout samples were subjected to SDS-PAGE. ab52633 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)](https://www.abcam.co.jp/ps/products/52/ab52633/Images/ab52633-65024-anti-gamma-synuclein-antibody-ep1539y-western-blot.jpg)
Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/2000 dilution + Human fetal brain lysate
Predicted band size: 13 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)](https://www.abcam.co.jp/ps/products/52/ab52633/Images/ab52633-65023-anti-gamma-synuclein-antibody-ep1539y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)ab52633 staining human brain in IHC-P.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)](https://www.abcam.co.jp/ps/products/52/ab52633/Images/ab52633-6-benefits-of-recombinant-antibodies.png)
Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab52633 は 4 報の論文で使用されています。
- Fei J et al. Inhibition of SNCG suppresses the proliferation of lung cancer cells induced by high glucose. Mol Med Rep 23:N/A (2021). PubMed: 33313952
- Chen Z et al. The down-regulation of SNCG inhibits the proliferation and invasiveness of human bladder cancer cell line 5637 and suppresses the expression of MMP-2/9. Int J Clin Exp Pathol 13:1873-1879 (2020). PubMed: 32782717
- Wang Y et al. Hypoxia-inducible lncRNA-AK058003 promotes gastric cancer metastasis by targeting ?-synuclein. Neoplasia 16:1094-106 (2014). WB . PubMed: 25499222
- Kim SH et al. Frequent expression of follicular dendritic cell markers in Hodgkin lymphoma and anaplastic large cell lymphoma. J Clin Pathol 66:589-96 (2013). IHC ; Human . PubMed: 23454725
