Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Sample
Human Cell lysate - other (lymphocytes)
Specification
lymphocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: ecl anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ecl
Bands
Specific: 15 kDa
Exposure
5 second(s)
Additional data
Additional Notes
Lane 1 from extreme left is human lymphocytes with no treatment, 2 & 3 - treated to aphidicolin, 4 & 5 - UV treatment
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 21 2013