Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/20000
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
I am not familiar with the distribution of this protein, hence
the "Inconclusive" rating.
The submitted image shows a highly differential pattern of positivity in seminiferous tubule cells : in some spermatagonium nuclei appear positive in others, only developing spermatocytes show an asymetric positive nuclear dot.
In some mature sperm cells, there appears to be a light nuclear positivity.
Abcam response
Thank you for your valuable feedback. Your stain pattern for ab2893 staining Rat testis is very similar to the one observed in PMID: 21310208. However, the staining that you observed was in the absence of DNA damage-inducing agents. We are unsure whether staining observed in untreated tissue is the expected staining for gammaH2AX. We hope this information is useful to our customers and we welcome further feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 May 29 2013