Anti-gamma Catenin 抗体 (ab15153)
Key features and details
- Rabbit polyclonal to gamma Catenin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-gamma Catenin antibody
gamma Catenin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to gamma Catenin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human gamma Catenin aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P14923 -
ポジティブ･コントロール
- Breast carcinoma
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15153の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
関連疾患
Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. -
配列類似性
Belongs to the beta-catenin family.
Contains 9 ARM repeats. -
細胞内局在
Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form. - Information by UniProt
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参照データベース
- Entrez Gene: 3728 Human
- Entrez Gene: 16480 Mouse
- Entrez Gene: 81679 Rat
- Omim: 173325 Human
- SwissProt: P14923 Human
- SwissProt: Q02257 Mouse
- SwissProt: Q6P0K8 Rat
- Unigene: 514174 Human
see all -
別名
- ARVD 12 antibody
- ARVD12 antibody
- Catenin (cadherin associated protein), gamma 80kDa antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody (ab15153)Human breast carcinoma stained with anti gamma catenin antibody ab15153 (1/100 for 10 min at RT). Gamma catenin staining is seen on the cell membrane and in the cytoplasm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody (ab15153)This image is courtesy of an anonymous Abreviewab15153 staining gamma Catenin in normal human skin tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% serum for 1 hour at 2°C; antigen retrival was by heat mediation in EDTA (pH9). The sample was incubated with primary antibody (1/100 in TBS + 1% BSA) at 21°C for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody (ab15153)ICC/IF image of ab15153 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab15153 は 6 報の論文で使用されています。
- Chen Y et al. Effects of differential distributed-JUP on the malignancy of gastric cancer. J Adv Res 28:195-208 (2021). PubMed: 33364056
- Ng R et al. Patient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation. JCI Insight 5:N/A (2019). PubMed: 31194698
- Boyden LM et al. Dominant de novo DSP mutations cause erythrokeratodermia-cardiomyopathy syndrome. Hum Mol Genet 25:348-57 (2016). PubMed: 26604139
- Guo Z et al. E-cadherin interactome complexity and robustness resolved by quantitative proteomics. Sci Signal 7:rs7 (2014). PubMed: 25468996
- Olsen PA et al. Implications of targeted genomic disruption of ß-catenin in BxPC-3 pancreatic adenocarcinoma cells. PLoS One 9:e115496 (2014). IP . PubMed: 25536063
- Rakha EA et al. Clinical and biological significance of E-cadherin protein expression in invasive lobular carcinoma of the breast. Am J Surg Pathol 34:1472-9 (2010). PubMed: 20871222
