Application
Western blot
Sample
Cow Cell lysate - whole cell (vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Treatment
1uM etoposide
Specification
vascular smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Anti-Rabbit IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
scanned with Licor Odyssey
Exposure
6 minute(s) and 0 second(s)
Additional data
Abcam response
ab11174 is yet to be tested with bovine samples however as there is high immunogenic sequence homology so it is predicted to cross- react. We welcome more feedback from other Scientist who are using this antibody with bovine samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 21 2017