Anti-galectin 9/Gal-9 抗体 (ab69630)
Key features and details
- Rabbit polyclonal to galectin 9/Gal-9
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-galectin 9/Gal-9 antibody
galectin 9/Gal-9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to galectin 9/Gal-9 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human galectin 9/Gal-9 aa 51-100 (internal sequence).
Database link: O00182 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69630の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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ICC/IF | (1) |
1/500 - 1/1000.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
組織特異性
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
配列類似性
Contains 2 galectin domains. -
ドメイン
Contains two homologous but distinct carbohydrate-binding domains. -
細胞内局在
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 3965 Human
- Entrez Gene: 16859 Mouse
- Entrez Gene: 25476 Rat
- Omim: 601879 Human
- SwissProt: O00182 Human
- SwissProt: O08573 Mouse
- SwissProt: P97840 Rat
- Unigene: 81337 Human
see all -
別名
- 36 kDa beta-galactoside-binding lectin antibody
- Ecalectin antibody
- Gal-9 antibody
see all
画像
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Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
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All lanes : Anti-galectin 9/Gal-9 antibody (ab69630) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Ab69630 recognizes the partial tagged recombinant Human galectin 9/Gal-9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab69630 は 28 報の論文で使用されています。
- Grabowska K et al. HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages. J Neurovirol 28:99-112 (2022). PubMed: 35175539
- Beyer S et al. Galectin-8 and -9 as prognostic factors for cervical cancer. Arch Gynecol Obstet 306:1211-1220 (2022). PubMed: 35377045
- Wu C et al. Single-cell characterization of malignant phenotypes and microenvironment alteration in retinoblastoma. Cell Death Dis 13:438 (2022). PubMed: 35523772
- Guo Q et al. Cancer cell intrinsic TIM-3 induces glioblastoma progression. iScience 25:105329 (2022). PubMed: 36325060
- Wang W et al. Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation. J Immunol 206:2692-2699 (2021). PubMed: 33963043