Rabbit polyclonal to Galectin 8 / Gal-8
Synthetic peptide corresponding to Human Galectin 8/ Gal-8 (internal sequence).
Extracts from 3T3 cells and human prostate carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS Without Mg2+ and Ca2+
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects bands of approximately 36 and 60 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Contains 2 galectin domains.
Contains two homologous but distinct carbohydrate-binding domains.
Information by UniProt
Gal 8 antibody
Western blot - Anti-Galectin 8 / Gal-8 antibody (ab69631)
All lanes : Anti-Galectin 8 / Gal-8 antibody (ab69631) at 1/500 dilution Lane 1 : extracts of 3T3 cells Lane 2 : extracts of 3T3 cells with immunising peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size: 36 kDa Observed band size: 36 kDa Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8 / Gal-8 antibody (ab69631)
Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using ab69631 at 1:50 dilution.
Left image un-treated.Right image treated with immunizing peptide.
This product has been referenced in:
Reticker-Flynn NE & Bhatia SN
Aberrant glycosylation promotes lung cancer metastasis through adhesion to galectins in the metastatic niche. Cancer Discov 5:168-81 (2015).
Read more (PubMed: 25421439) »
et al. A combinatorial extracellular matrix platform identifies cell-extracellular matrix interactions that correlate with metastasis. Nat Commun 3:1122 (2012).
Read more (PubMed: 23047680) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"