Anti-Galectin 3 抗体 (ab31707)
Key features and details
- Rabbit polyclonal to Galectin 3
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Galectin 3 antibody
Galectin 3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Galectin 3 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab76245.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog, Pig -
免疫原
Synthetic peptide corresponding to Human Galectin 3 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab31706) -
ポジティブ・コントロール
- Recombinant human Galectin 3 protein (ab50236) can be used as a positive control in WB. HeLa (Human epithelial carcinoma cell line) Whole Cell and Nuclear Lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31707の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
組織特異性
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
配列類似性
Contains 1 galectin domain. -
細胞内局在
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 3958 Human
- Omim: 153619 Human
- SwissProt: P38486 Dog
- SwissProt: P17931 Human
- Unigene: 531081 Human
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別名
- 35 kDa lectin antibody
- Carbohydrate binding protein 35 antibody
- Carbohydrate-binding protein 35 antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Galectin 3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab31707 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab31707 was shown to specifically recognize Galectin 3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Galectin 3 knockout samples were examined. Wild-type and Galectin 3 knockout samples were subjected to SDS-PAGE. Ab31707 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ICC/IF image of ab31707 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31707, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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All lanes : Anti-Galectin 3 antibody (ab31707) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
We also see a very faint band at 52 kDa in the HeLa Whole cell lysate, we are uncertain as to the identity of this band.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab31707 は 10 報の論文で使用されています。
- Millarte V et al. Rabaptin5 targets autophagy to damaged endosomes and Salmonella vacuoles via FIP200 and ATG16L1. EMBO Rep 23:e53429 (2022). PubMed: 34704340
- Koerver L et al. The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage. EMBO Rep 20:e48014 (2019). PubMed: 31432621
- Coppin L et al. Galectin-3 is a non-classic RNA binding protein that stabilizes the mucin MUC4 mRNA in the cytoplasm of cancer cells. Sci Rep 7:43927 (2017). PubMed: 28262838
- Zheng J et al. Galectin-3 induced by hypoxia promotes cell migration in thyroid cancer cells. Oncotarget 8:101475-101488 (2017). PubMed: 29254179
- Okawa S et al. Proteome and Secretome Characterization of Glioblastoma-Derived Neural Stem Cells. Stem Cells 35:967-980 (2017). PubMed: 27870168
- Truchan HK et al. Type II Secretion Substrates of Legionella pneumophila Translocate Out of the Pathogen-Occupied Vacuole via a Semipermeable Membrane. MBio 8:N/A (2017). ICC/IF . PubMed: 28634242
- Ma Z et al. Galectin-3 Inhibition Is Associated with Neuropathic Pain Attenuation after Peripheral Nerve Injury. PLoS One 11:e0148792 (2016). WB . PubMed: 26872020
- DE Oliveira JT et al. Differential expression of galectin-1 and galectin-3 in canine non-malignant and malignant mammary tissues and in progression to metastases in mammary tumors. Anticancer Res 34:2211-21 (2014). PubMed: 24778023
- Petropolis DB et al. A new human 3D-liver model unravels the role of galectins in liver infection by the parasite Entamoeba histolytica. PLoS Pathog 10:e1004381 (2014). ICC/IF ; Human . PubMed: 25211477
- Cui GQ et al. Proteomic analysis of meningiomas. Acta Neurol Belg 114:187-94 (2014). WB ; Human . PubMed: 24085542