Anti-Galectin 3 抗体 (ab227249)
Key features and details
- Rabbit polyclonal to Galectin 3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Galectin 3 antibody
Galectin 3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Galectin 3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Rhesus monkey -
免疫原
Recombinant fragment within Human Galectin 3 (internal sequence). The exact sequence is proprietary.
Database link: P17931 -
ポジティブ・コントロール
- WB: HeLa cell extract; A549, H1299, HCT 116 and MCF7 whole cell lysates; Galectin 3 transfected HEK-293T cell lysate; rat colon lysate. ICC/IF: MCF7 cells. IHC-P: HBL435 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227249の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (2) |
1/100 - 1/1000.
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WB |
1/5000 - 1/20000. Predicted molecular weight: 26 kDa.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/20000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
組織特異性
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
配列類似性
Contains 1 galectin domain. -
細胞内局在
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 3958 Human
- Entrez Gene: 83781 Rat
- Entrez Gene: 697290 Rhesus monkey
- Omim: 153619 Human
- SwissProt: P17931 Human
- SwissProt: P08699 Rat
- Unigene: 531081 Human
- Unigene: 764 Rat
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別名
- 35 kDa lectin antibody
- Carbohydrate binding protein 35 antibody
- Carbohydrate-binding protein 35 antibody
see all
画像
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All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Galectin 3 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa -
Paraffin-embedded HBL435 xenograft tissue stained for Galectin 3 using ab227249 at 1/500 dilution in immunohistochemical analysis.
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Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Galectin 3 (green) using ab227249 at 1/1000 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12% SDS-PAGE gel.
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All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Galectin 3 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12% SDS PAGE gel-
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Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution + Rat colon lysate at 50 µg
Predicted band size: 26 kDa12 % SDS-PAGE gel.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227249 は論文での使用が確認できていません。