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  1. Link

    galectin-3-antibody-a3a12-ab2785.pdf

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Neuroscience Cell Adhesion Proteins Nuclear Proteins
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Anti-Galectin 3 抗体 [A3A12] (ab2785)

  • Datasheet
Reviews (13)Q&A (6)References (39)

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Abpromise

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Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

Key features and details

  • Mouse monoclonal [A3A12] to Galectin 3
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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タンパク質
Recombinant human Galectin 3 protein (Active) (ab50236)

関連製品

製品の概要

  • 製品名

    Anti-Galectin 3 antibody [A3A12]
    Galectin 3 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [A3A12] to Galectin 3
  • 由来種

    Mouse
  • 特異性

    By Western blot, this antibody detects an ~30 kDa protein representing Galectin 3 from Jurkat cells transfected with human Galectin 3. Immunohistochemical staining of Galectin 3 in rat olfactory bulb yields a pattern consistent with nuclear and plasma membrane staining.
  • アプリケーション

    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Galectin 3.

  • エピトープ

    The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.
  • ポジティブ・コントロール

    • WB: HeLa, MCF7 whole cell lysates. IHC-P: Human ovarian, colon and mouse colon tissue. ICC: HeLa, MCF7 and NIH-3T3 cells.
  • 特記事項

     

    This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 一次抗体 備考

    This antibody has been shown to potentiate the binding of Galectin 3 to IgG.
  • ポリ/モノ

    モノクローナル
  • クローン名

    A3A12
  • アイソタイプ

    IgG1
  • 研究分野

    • Neuroscience
    • Cell Adhesion Proteins
    • Nuclear Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Galectin 3 protein (Active) (ab50236)

アプリケーション

Our Abpromise guarantee covers the use of ab2785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ICC/IF 1/100 - 1/200.
IHC-P 1/20 - 1/200.

ターゲット情報

  • 機能

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 組織特異性

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 配列類似性

    Contains 1 galectin domain.
  • 細胞内局在

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Target information above from: UniProt accession P17931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3958 Human
    • Entrez Gene: 16854 Mouse
    • Omim: 153619 Human
    • SwissProt: P17931 Human
    • SwissProt: P16110 Mouse
    • Unigene: 531081 Human
    • Unigene: 248615 Mouse
    • 別名

      • 35 kDa lectin antibody
      • Carbohydrate binding protein 35 antibody
      • Carbohydrate-binding protein 35 antibody
      • CBP 35 antibody
      • CBP35 antibody
      • Gal-3 antibody
      • GAL3 antibody
      • Galactose-specific lectin 3 antibody
      • Galactoside-binding protein antibody
      • GALBP antibody
      • Galectin 3 internal gene,included antibody
      • Galectin-3 antibody
      • Galectin3 antibody
      • GALIG antibody
      • GBP antibody
      • IgE binding protein antibody
      • IgE-binding protein antibody
      • L 31 antibody
      • L 34 antibody
      • L-31 antibody
      • L-34 galactoside-binding lectin antibody
      • L31 antibody
      • Laminin-binding protein antibody
      • Lectin L-29 antibody
      • Lectin, galactose binding, soluble 3 antibody
      • LEG3_HUMAN antibody
      • LGALS2 antibody
      • LGALS3 antibody
      • MAC 2 antigen antibody
      • Mac-2 antibody
      • Mac-2 antigen antibody
      • MAC2 antibody
      • Macrophage galactose-specific lectin antibody
      • MGC105387 antibody
      see all

    画像

    • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Predicted band size: 26 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis of human ovarian carcinoma tissue (right) labeling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Galectin 3 monoclonal antibody (Product # MA1-940) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis (formalin/PFA-fixed paraffin-embedded sections) human colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis of (formalin/PFA-fixed paraffin-embedded sections) mouse colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by . To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Galectin 3 with ab2785. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2785, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (39)

    ab2785 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2785 は 39 報の論文で使用されています。

    • Kim JY  et al. Protective Effects of SPA0355, a Thiourea Analogue, Against Lipopolysaccharide-Induced Acute Kidney Injury in Mice. Antioxidants (Basel) 9:N/A (2020). PubMed: 32635491
    • Li T  et al. Galectin-3 Mediates Endothelial-to-Mesenchymal Transition in Pulmonary Arterial Hypertension. Aging Dis 10:731-745 (2019). PubMed: 31440380
    • Volarevic V  et al. Galectin 3 protects from cisplatin-induced acute kidney injury by promoting TLR-2-dependent activation of IDO1/Kynurenine pathway in renal DCs. Theranostics 9:5976-6001 (2019). PubMed: 31534532
    • Saleh A  et al. A novel 3D nanofibre scaffold conserves the plasticity of glioblastoma stem cell invasion by regulating galectin-3 and integrin-ß1 expression. Sci Rep 9:14612 (2019). PubMed: 31601895
    • Fan J  et al. Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine ? lyase S-sulfhydration in ApoE-knockout atherosclerotic mice. Br J Pharmacol 176:3180-3192 (2019). PubMed: 31140595
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 19 Abreviews or Q&A

    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 09 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice Hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice Hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    投稿 Apr 02 2019

    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Mice Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    投稿 Feb 20 2019

    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (DU145)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    25 µg
    Specification
    DU145
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    投稿 Mar 31 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    投稿 Mar 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (PC-3 prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    PC-3 prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    投稿 Feb 21 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Tumors slides)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Tumors slides
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mr. Thiago Vargas

    Verified customer

    投稿 Apr 05 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Galectin 3 [A3A12] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Tissue sections (Retina)
    Specification
    Retina
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 17 2014

    Question

    What is the buffer composition of PBS?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2013

    Answer

    Thank you for contacting us.

    We use a PBS pH 7,4 +/- 0.1 containing Potassium as well as Sodium phosphate, KCL and NaCl.

    For a liter of a 20x stock we use: Potassium phosphate monobasic 4,8 gNaCl 160 gKCL 4 gSodium phosphate dibasic 28,8 g In the antibody the buffer is used as a 1 x dilution.

    I can confirm that it does not contain Mg2+ and Ca2+ ions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2013

    Immunocytochemistry abreview for Anti-Galectin 3 antibody [A3A12]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Kelly Neuroblastoma cell line)
    Specification
    Kelly Neuroblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 28 2012

    1-10 of 19 Abreviews or Q&A

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    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
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