アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    galectin-3-antibody-a3a12-ab2785.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Adhesion Proteins Nuclear Proteins
Share by email

Anti-Galectin 3 抗体 [A3A12] (ab2785)

  • Datasheet
  • SDS
Reviews (14)Q&A (6)References (49)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

Key features and details

  • Mouse monoclonal [A3A12] to Galectin 3
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
Product image
Anti-Galectin 3 antibody [EP2775Y] - BSA and Azide free (ab197544)
アッセイ
Product image
HDL Uptake Assay Kit (Fluorometric) (ab204717)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Galectin 3 antibody [A3A12]
    Galectin 3 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [A3A12] to Galectin 3
  • 由来種

    Mouse
  • 特異性

    By Western blot, this antibody detects an ~30 kDa protein representing Galectin 3 from Jurkat cells transfected with human Galectin 3. Immunohistochemical staining of Galectin 3 in rat olfactory bulb yields a pattern consistent with nuclear and plasma membrane staining.
  • アプリケーション

    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Galectin 3.

  • エピトープ

    The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.
  • ポジティブ・コントロール

    • WB: HeLa, MCF7 whole cell lysates. IHC-P: Human ovarian, colon and mouse colon tissue. ICC: HeLa, MCF7 and NIH-3T3 cells.
  • 特記事項

     

    This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 一次抗体 備考

    This antibody has been shown to potentiate the binding of Galectin 3 to IgG.
  • ポリ/モノ

    モノクローナル
  • クローン名

    A3A12
  • アイソタイプ

    IgG1
  • 研究分野

    • Neuroscience
    • Cell Adhesion Proteins
    • Nuclear Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Galectin 3 protein (Active) (ab50236)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2785の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ICC/IF (5)
1/100 - 1/200.
IHC-P (2)
1/20 - 1/200.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ICC/IF
1/100 - 1/200.
IHC-P
1/20 - 1/200.

ターゲット情報

  • 機能

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 組織特異性

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 配列類似性

    Contains 1 galectin domain.
  • 細胞内局在

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Target information above from: UniProt accession P17931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3958 Human
    • Entrez Gene: 16854 Mouse
    • Omim: 153619 Human
    • SwissProt: P17931 Human
    • SwissProt: P16110 Mouse
    • Unigene: 531081 Human
    • Unigene: 248615 Mouse
    • 別名

      • 35 kDa lectin antibody
      • Carbohydrate binding protein 35 antibody
      • Carbohydrate-binding protein 35 antibody
      • CBP 35 antibody
      • CBP35 antibody
      • Gal-3 antibody
      • GAL3 antibody
      • Galactose-specific lectin 3 antibody
      • Galactoside-binding protein antibody
      • GALBP antibody
      • Galectin 3 internal gene,included antibody
      • Galectin-3 antibody
      • Galectin3 antibody
      • GALIG antibody
      • GBP antibody
      • IgE binding protein antibody
      • IgE-binding protein antibody
      • L 31 antibody
      • L 34 antibody
      • L-31 antibody
      • L-34 galactoside-binding lectin antibody
      • L31 antibody
      • Laminin-binding protein antibody
      • Lectin L-29 antibody
      • Lectin, galactose binding, soluble 3 antibody
      • LEG3_HUMAN antibody
      • LGALS2 antibody
      • LGALS3 antibody
      • MAC 2 antigen antibody
      • Mac-2 antibody
      • Mac-2 antigen antibody
      • MAC2 antibody
      • Macrophage galactose-specific lectin antibody
      • MGC105387 antibody
      see all

    画像

    • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
      Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 6 : A431 (human epidermoid carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG (H+L) (HRP) at 1/4000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?
      Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.



      Whole cell extracts were electrophoresed using a 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a Nitrocellulose membrane using a dry blotting system.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis of human ovarian carcinoma tissue (right) labeling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Galectin 3 monoclonal antibody (Product # MA1-940) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x

    • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)
      All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : Wild-Type HeLa (human epithelial cell line from cervix adenocarcinoma) membrane extract
      Lane 2 : HeLa CAS9 membrane extract
      Lane 3 : HeLa LGALS3 knockout membrane extract

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG (H+L) (HRP) at 1/4000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa



      Knockout of LGALS3 was achieved by CRISPR-Cas9 genome editing using Lentiviral sgRNA and Cas9 Lentivirus. The samples were electrophoresed using 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a nitrocellulose membrane using a dry blotting system.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis (formalin/PFA-fixed paraffin-embedded sections) human colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunohistochemical analysis of (formalin/PFA-fixed paraffin-embedded sections) mouse colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by . To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

      Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (49)

    ab2785 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2785 は 49 報の論文で使用されています。

    • Liu F  et al. Insulin Receptor Substrate p53 Ameliorates High-Glucose-Induced Activation of NF-?B and Impaired Mobility of HUVECs. Biomed Res Int 2021:3210586 (2021). PubMed: 33506012
    • Marhuenda E  et al. Glioma stem cells invasive phenotype at optimal stiffness is driven by MGAT5 dependent mechanosensing. J Exp Clin Cancer Res 40:139 (2021). PubMed: 33894774
    • Chen Z  et al. Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway. Mediators Inflamm 2021:6639252 (2021). PubMed: 33927570
    • van Veen S  et al. ATP13A2 deficiency disrupts lysosomal polyamine export. Nature 578:419-424 (2020). PubMed: 31996848
    • Shimba Y  et al. ß-Aminoisobutyric Acid Suppresses Atherosclerosis in Apolipoprotein E-Knockout Mice. Biol Pharm Bull 43:1016-1019 (2020). PubMed: 32475911
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 20 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human prostate tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Human prostate tissue
    Blocking step
    Blocking according to ab183290 IHC Kit: M&R&G on human tissue as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Apr 11 2022

    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 09 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice Hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice Hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Apr 02 2019

    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Mice Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Feb 20 2019

    Western blot abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (DU145)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    25 µg
    Specification
    DU145
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Mar 31 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Mar 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (PC-3 prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    PC-3 prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Feb 21 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Galectin 3 antibody [A3A12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Tumors slides)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Tumors slides
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mr. Thiago Vargas

    Verified customer

    投稿 Apr 05 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Galectin 3 [A3A12] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Tissue sections (Retina)
    Specification
    Retina
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 17 2014

    Question

    What is the buffer composition of PBS?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2013

    Answer

    Thank you for contacting us.

    We use a PBS pH 7,4 +/- 0.1 containing Potassium as well as Sodium phosphate, KCL and NaCl.

    For a liter of a 20x stock we use: Potassium phosphate monobasic 4,8 gNaCl 160 gKCL 4 gSodium phosphate dibasic 28,8 g In the antibody the buffer is used as a 1 x dilution.

    I can confirm that it does not contain Mg2+ and Ca2+ ions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2013

    1-10 of 20 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.