Anti-Galectin 13/PLAC8 + Galectin-16 抗体 [EPR23383-89] (ab272716)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] -
製品の詳細
Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human placenta tissue lysate; His-tagged human Galectin 13/PLAC8 recombinant protein; His-lif tagged human Galectin 16 recombinant protein (aa 1-141). IHC-P: Human placenta tissue. IP: Human placenta tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23383-89 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272716の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
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特記事項 |
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WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
画像
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All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 10527825, 19497882).
Negative control: Human skeletal muscle (PMID: 10527825).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
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All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution
Lane 1 : His-tagged human Galectin 13/PLAC8 recombinant protein (aa 1-139, ab124309), 20 ng
Lane 2 : His-lif tagged human Galectin 16 recombinant protein (aa 1-141) 5 µL
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 16 kDa
Observed band size: 15,55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This product has cross reaction with Galectin 16.
Sample loaded onto lane 2 was extract of HEK293 expressing Galectin 16, which contains a lif tag (40 kDa).
Exposure time: 70 seconds.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Galectin 13+Galectin 16 with ab272716 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta (PMID: 25191322). The section was incubated with ab272716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Galectin 13+Galectin 16 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab272716 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Human placenta tissue lysate 10µg
Lane 2: ab272716 IP in human placenta tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272716 in human placenta tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab272716 は論文での使用が確認できていません。