リコンビナントRabMAb

Anti-Galectin 13/PLAC8 + Galectin-16 抗体 [EPR23383-89] (ab272716)

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Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
  • Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89]

  • 製品の詳細

    Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human placenta tissue lysate; His-tagged human Galectin 13/PLAC8 recombinant protein; His-lif tagged human Galectin 16 recombinant protein (aa 1-141). IHC-P: Human placenta tissue. IP: Human placenta tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23383-89

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
IP
Human
WB
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
特記事項
WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.

画像

  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 10527825, 19497882).

    Negative control: Human skeletal muscle (PMID: 10527825).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution

    Lane 1 : His-tagged human Galectin 13/PLAC8 recombinant protein (aa 1-139, ab124309), 20 ng
    Lane 2 : His-lif tagged human Galectin 16 recombinant protein (aa 1-141) 5 µL

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 16 kDa
    Observed band size: 15/55 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This product has cross reaction with Galectin 16.

    Sample loaded onto lane 2 was extract of HEK293 expressing Galectin 16, which contains a lif tag (40 kDa). 

    Exposure time: 70 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Galectin 13+Galectin 16 with ab272716 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta (PMID: 25191322). The section was incubated with ab272716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

    Galectin 13+Galectin 16 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab272716 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Human placenta tissue lysate 10µg

    Lane 2: ab272716 IP in human placenta tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272716 in human placenta tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

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