Application
Western blot
Sample
Rat Cell lysate - whole cell (Mesenchymal Stem Cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mesenchymal Stem Cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-Goat IgG H&L
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 13 kDa Non-specific: 16 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Yen You Nick Chen
Verified customer
投稿 Dec 21 2016