Application
Immunohistochemistry (Frozen sections)
Sample
Fruit fly (Drosophila melanogaster) Tissue sections (eye imaginal disc)
Specification
eye imaginal disc
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonX
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: ~20°C
Other product details
Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Laboratory Affinipure
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Dilution
1/500
Additional data
Additional Notes
eye gone Gal4 eye discs were unsuccessfully stained using the rabbit anti-GAL4 at the following concentrations:
1:50, 1:200, 1:500 and 1:1000
1:50, 1:200, 1:500 and 1:1000
Abcam response
Thank you for your valuable feedback and for participating in our Abreview testing discount program. We are sorry to hear that this antibody has not given you good results in immunohistochemistry frozen sections using fruit fly sample. This antibody has not been tested with this species nor application before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Vilaiwan Fernandes
Verified customer
投稿 Nov 16 2011