Anti-GABARAP 抗体 (ab1398)
製品の概要
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製品名
Anti-GABARAP antibody
GABARAP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GABARAP -
由来種
Rabbit -
特異性
This antibody recognizes human GABARAP (14 kD). -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
RSEGEKIRKKYPDRVPV
, corresponding to amino acids 15-31 of Human GABARAP.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ELISA | 1/1000 - 1/5000. | |
WB | 1/500 - 1/1000. |
ターゲット情報
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機能
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. -
組織特異性
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the ATG8 family. -
翻訳後修飾
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II. -
細胞内局在
Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 11337 Human
- Entrez Gene: 56486 Mouse
- Omim: 605125 Human
- SwissProt: O95166 Human
- SwissProt: Q9DCD6 Mouse
- Unigene: 647421 Human
- Unigene: 272460 Mouse
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別名
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
see all
画像
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Anti-GABARAP antibody (ab1398) at 1 µg/ml + Macrophage whole cell lysate at 80 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 14 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Li L et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . Read more (PubMed: 28534953) »
- Ning HJ et al. Propofol reduces hypoxia-induced autophagic cell death through downregulating HIF 1a in alveolar epithelial type II cells of rats. Mol Med Rep 16:1509-1515 (2017). Read more (PubMed: 28586054) »