アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Called to discuss problems using ab72448.
Asked on Mar 30 2012
Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
As we discussed on the phone, I am including a brief questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.
Could you tell me the lysis buffer that you have used?
Have you reduced and denatured your samples? Which method/buffers did you use?
How much total protein did you use per well?
Did you load a loading control? Which LC did you use? What were the results?
Did you load a positive control? Which PC did you use? What were the results?
What membrane type did you transfer to?
What blocking steps did you use?
What dilutions of this product did you use? How long and at what temperature was this antibody incubated?
What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?
Have you run a no primary antibody control blot? What were the results?
What is the wash buffer that you use?
Could you provide the Abcam with any images you may have?
Finally, would you be able to provide the order confirmation number or PO number used to purchase this?
Answered on Mar 30 2012