製品の概要

  • 製品名
    Anti-GABA Transporter 1 / GAT 1 antibody
    GABA Transporter 1 / GAT 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GABA Transporter 1 / GAT 1
  • 由来種
    Rabbit
  • 特異性
    Reacts specifically with a 72 kDa protein.
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
    Database link: P23978

  • ポジティブ・コントロール
    • IHC-Fr: Mouse retinal tissue. WB: Rat hippocampus, spinal cord and brain tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500.
IHC-Fr 1/50 - 1/500.
WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 配列類似性
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • ドメイン
    The PDZ domain-binding motif is involved in the interaction with MPP5.
  • 細胞内局在
    Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

画像

  • ab426 staining the GABA transporter 1 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2000 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

参考文献

This product has been referenced in:
  • Qian Z  et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). Read more (PubMed: 29693164) »
  • Ding XD  et al. Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Exp Ther Med 13:2783-2786 (2017). Read more (PubMed: 28588664) »
See all 17 Publications for this product

レビューと Q&A

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1-3 of 3 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization
No
Specification
Brain_Region B
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
formalin

Poonam Rani

Verified customer

投稿 Apr 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
10 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Mrs. Olga Cravetchi

Verified customer

投稿 Nov 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina sections)
Specification
mouse retina sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Mr. Kevin Jin

Verified customer

投稿 Nov 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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