製品の概要
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製品名Anti-GABA Transporter 1 / GAT 1 antibody
GABA Transporter 1 / GAT 1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to GABA Transporter 1 / GAT 1
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由来種Rabbit
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特異性Reacts specifically with a 72 kDa protein.
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アプリケーション適用あり: IHC-P, IHC-Fr, WB, IHC-FoFrmore details
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種交差性交差種: Mouse, Rat, Chicken
交差が予測される動物種: Human
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免疫原
Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
Database link: P23978 -
ポジティブ･コントロール
- IHC-Fr: Mouse retinal tissue. WB: Rat hippocampus, spinal cord and brain tissue lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーConstituent: Whole serum
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精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/50 - 1/500. | |
| IHC-Fr | 1/50 - 1/500. | |
| WB | 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa). | |
| IHC-FoFr | Use at an assay dependent concentration. |
ターゲット情報
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機能Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
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配列類似性Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
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ドメインThe PDZ domain-binding motif is involved in the interaction with MPP5.
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細胞内局在Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
- Information by UniProt
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参照データベース
- Entrez Gene: 415960 Chicken
- Entrez Gene: 6529 Human
- Entrez Gene: 232333 Mouse
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P31648 Mouse
- SwissProt: P23978 Rat
see all -
別名
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)This image is courtesy of an Abreview submitted by Kevin Jinab426 staining the GABA transporter 1 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2000 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody. -
Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 74 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 seconds
GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
参考文献
This product has been referenced in:
- Qian Z et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). Read more (PubMed: 29693164) »
- Ding XD et al. Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Exp Ther Med 13:2783-2786 (2017). Read more (PubMed: 28588664) »
レビューと Q&A
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