Anti-GABA Transporter 1 / GAT 1 抗体 (ab426)
Key features and details
- Rabbit polyclonal to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-GABA Transporter 1 / GAT 1 antibody
GABA Transporter 1 / GAT 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GABA Transporter 1 / GAT 1 -
由来種
Rabbit -
特異性
Reacts specifically with a 72 kDa protein. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Human -
免疫原
Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
Database link: P23978 -
ポジティブ・コントロール
- WB: Rat Hippocampus Tissue Lysate; Rat Spinal Cord Tissue Lysate; Rat Brain Tissue Lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab426の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
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特記事項 |
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WB
1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa). |
ターゲット情報
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機能
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
配列類似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
ドメイン
The PDZ domain-binding motif is involved in the interaction with MPP5. -
細胞内局在
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
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参照データベース
- Entrez Gene: 6529 Human
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P23978 Rat
- Unigene: 443874 Human
- Unigene: 10035 Rat
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別名
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
画像
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All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
データシートおよび資料
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Datasheet download
参考文献 (36)
ab426 は 36 報の論文で使用されています。
- Zhang D et al. Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing. FEBS Open Bio 12:864-876 (2022). PubMed: 35147292
- Lin Y et al. Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery. Theranostics 11:3760-3780 (2021). PubMed: 33664860
- Wang S et al. Decreased GABAergic signaling, fewer parvalbumin-, somatostatin- and calretinin-positive neurons in brain of a rat model of simulated transport stress. Res Vet Sci 134:86-95 (2021). PubMed: 33360121
- Chen M et al. Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models. Mol Neurodegener 16:14 (2021). PubMed: 33663578
- Balzano T et al. Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage. Biomedicines 9:N/A (2021). PubMed: 34440206