Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample
Chicken Tissue sections (chicken hindbrain)
Specification
chicken hindbrain
Permeabilization
Yes - 0.1% PBS-TritonX
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1 % PBS-TritonX + 3% BSA
Dilution
1/1000
Secondary antibody
Name
Abcam antibody:Goat Anti-Guinea pig IgG H&L (Alexa Fluor 568)
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Karli Montague
Verified customer
投稿 Mar 26 2014