Anti-GABA 抗体 (ab17413)

I can confirm that ab17413 anti-GABA antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml.

Thank you for bringing this to our attention. We have not tested the antibody on free-floating sections but your protocol is correct, assuming 10 minutes for permeabilization of these 50um sections was sufficient. We have one other complaint about this lot from a customer who was trying to stain similar samples. We do have a different lot now. If you like, I will be happy to send a vial as a free replacement, or a different antibody altogether, such as the rabbit polyclonal ab8891:

Click here (or use the following: https://www.abcam.com/GABA-antibody-ab8891.html).

Thank you for contacting us.

This antibody has been tested on GABAergic interneurons of mammalian samples, but we do not have testing or reports using hESC.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for providing some further useful information.

Both antibodies would be suitable for the immunostaining. However, it seems that ab17413 is more popular amongst our customers since it has been characterized in a number of applications. Moreover, we have one published feedback (see Abreview) and several specific published papers are available.

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for contacting Abcam regarding ab17413. Regarding the tissue fixation, I have confirmed with the laboratory that yes, the combination of 4% paraformaldehyde / glutaraldehyde fixation is typically used in successful IHC staining.  Please find the protocol  is below: Immunohistochemistry Protocol: Fixation:  Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde give good results. 1. Tissues are fixed with 0.1M phosphate buffer, pH 6.5, 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate. Tissue post-fixed overnight. 2. Cut sections in 50um thickness. 3. Incubate in 0.05M Tris buffer, pH 6.5 for 3 hrs. 4. Sections are incubated for up to 18-24 hours in ab17413 diluted with PBS, 0.1% sodium azide, 0.2% Triton X-100, 1% normal goat serum. 5. Fluorescein conjugated antibody or PAP may be used as the secondary reagent. I hope this information is helpful.  Please do not hesitate to contact me if you have any additional questions.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimize the results from ab8891. I would also appreciate if you can confirm some further details:   1. I can recommend to try antigen retrieval as the tissue has been in PFA overnight, which is quite a long time. 2. Could you provide further details of the permebilization? I can suggest to try 0.1% Triton for 10 minutes only as Triton is quite a strong detergent, and can disrupt protein structure. 3. I can suggest to Use 0.2% Tween (a more gentle detergent) in the antibody dilution buffer, wash buffer and blocking buffer. 4. We recommend not to mix blocking agents in an experiment. Try 5% goat serum only. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.