Anti-GABA A Receptor beta 3/GABRB3 抗体 [N87/25] (ab98968)
Key features and details
- Mouse monoclonal [N87/25] to GABA A Receptor beta 3/GABRB3
- Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25]
GABA A Receptor beta 3/GABRB3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N87/25] to GABA A Receptor beta 3/GABRB3 -
由来種
Mouse -
特異性
Does not cross react with GABA A Receptor Beta 2 or Beta 1. -
アプリケーション
適用あり: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Orangutan -
免疫原
Fusion protein corresponding to Mouse GABA A Receptor beta 3/GABRB3 aa 350-450.
Database link: P63080 -
ポジティブ・コントロール
- WB: Rat brain lysate. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse brain tissue.
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特記事項
The clone number has been updated from S87-25 to N87/25, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N87/25 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab98968の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
1/1000.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 55 kDa.
Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
特記事項 |
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IHC-Fr
1/1000. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 55 kDa. Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
ターゲット情報
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機能
Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. -
関連疾患
Epilepsy, childhood absence 5 -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. -
細胞内局在
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2562 Human
- Entrez Gene: 14402 Mouse
- Entrez Gene: 24922 Rat
- Omim: 137192 Human
- SwissProt: P28472 Human
- SwissProt: P63080 Mouse
- SwissProt: P63079 Rat
- Unigene: 302352 Human
see all -
別名
- ECA5 antibody
- GABA alpha receptor beta-2 subunit antibody
- GABA(A) receptor subunit beta-3 antibody
see all
画像
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Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 55 kDaBlock: 1.5% BSA for 30 minutes at RT.
Incubation with primary antibody for 2 hours at RT.
Incubation with secondary antibody for 1 hour at RT.
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ab98968 staining GABA A Receptor beta 3/GABRB3 in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
Tissue was fixed in 10% formalin for 12-24 hours at RT. Primary Antibody: ab98968 at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
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Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab98968 (red line).
The cells were fixed with 4% paraformaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 minutes at 22ºC.
The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab98968 は 14 報の論文で使用されています。
- Menzikov SA et al. Physiological Role of ATPase for GABAA Receptor Resensitization. Int J Mol Sci 23:N/A (2022). PubMed: 35628132
- Chu MC et al. Generational synaptic functions of GABAA receptor β3 subunit deteriorations in an animal model of social deficit. J Biomed Sci 29:51 (2022). PubMed: 35821032
- Liu Q et al. Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABAA R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh) 8:e2102079 (2021). PubMed: 34263559
- Farmer GE et al. AT1a-dependent GABAA inhibition in the MnPO following chronic intermittent hypoxia. Am J Physiol Regul Integr Comp Physiol 321:R469-R481 (2021). PubMed: 34189959
- Victor AK et al. Molecular Changes in Prader-Willi Syndrome Neurons Reveals Clues About Increased Autism Susceptibility. Front Mol Neurosci 14:747855 (2021). PubMed: 34776864