Anti-GABA A Receptor alpha 1 抗体 [EPR5401(2)] (ab151573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)]
GABA A Receptor alpha 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human GABA A Receptor alpha 1 aa 1-100. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human fetal brain, Human lung and Human cerebellum lysates; Human brain prepared in 1%SDS Hot lysis method. IHC-P: Human brain, colon, kidney, Ovarian carcinoma, spleen, cerebrum and glioma tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5401(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151573の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
We recommend to try 1% SDS Hot lysis prepare method to get desired WB results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use 1/50 - 1/100 |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). We recommend to try 1% SDS Hot lysis prepare method to get desired WB results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use 1/50 - 1/100 |
ターゲット情報
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機能
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
関連疾患
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
細胞内局在
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2554 Human
- Omim: 137160 Human
- SwissProt: P14867 Human
- Unigene: 175934 Human
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別名
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling GABA A Receptor alpha 1 with Purified ab151573 at 1:1000 dilution (0.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution
Lane 1 : Human brain prepared in RIPA lysis method
Lane 2 : Human brain prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 40 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 0.7 µg/ml (purified)
Lane 1 : Human brain lysates
Lane 2 : Human lung lysates - negative control
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 52 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Please note: Lysates prepared in 1%SDS Hot lysis method.According to the paper (PMID: 29467616), brain is positive and lung is negative tissue.
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDaPlease note: Lysates prepared in 1%SDS Hot lysis method.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.
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Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151573 showing -ve staining.
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Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151573 showing -ve staining.
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151573 showing -ve staining.
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Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab151573 showing -ve staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab151573 は 1 報の論文で使用されています。
- Tabassum S et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). PubMed: 30872728