リコンビナントRabMAb

Anti-GABA A Receptor alpha 1 抗体 [EPR23539-255] - BSA and Azide free (ab272398)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)
  • Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)
  • Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free
    GABA A Receptor alpha 1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    IP
    Mouse
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse and rat cerebrum tissue. WB: Mouse and rat brain, cerebellum and hippocampus tissue lysate. Human brain and cerebellum tissue lysate. IP: Mouse cerebellum tissue lysate.

  • 特記事項

    ab272398 is the carrier-free version of ab252430. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Rat
IP
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.

    • ターゲット情報

    • 機能

      GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

    • 関連疾患

      Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
      Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.

    • 配列類似性

      Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

    • 細胞内局在

      Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • ECA4 antibody
      • EIEE19 antibody
      • EJM antibody
      • EJM5 antibody
      • Gaba receptor alpha 1 polypeptide antibody
      • GABA(A) receptor antibody
      • GABA(A) receptor subunit alpha 1 antibody
      • GABA(A) receptor subunit alpha-1 antibody
      • GABA(A) receptor, alpha 1 antibody
      • GABRA 1 antibody
      • GABRA1 antibody
      • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
      • Gamma aminobutyric acid A receptor alpha 1 antibody
      • Gamma aminobutyric acid receptor subunit alpha 1 antibody
      • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
      • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
      • GBRA1_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrumis observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252430).

    • Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)
      Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)

      GABA A Receptor alpha 1 was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10µg with ab252430 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252430 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse cerebellum tissue lysate 10µg.

      Lane 2: ab252430 IP in mouse cerebellum tissue lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252430 in mouse cerebellum tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 33 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252430).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum is observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252430).

    • Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)
      Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] - BSA and Azide free (ab272398)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272398 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272398 は論文での使用が確認できていません。

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