Anti-GAA 抗体 (ab102815)

That seems like a good idea.

I would possible also suggest reducing the washing conditions used slightly, using 3x5 minute washes with TBST rather than extended periods.

Please do let me know how you get on.

Thank you for contacting us.

I now have some further information in regards to how the western blot presented on the datasheet of Anti-GAA antibody (ab102815) was performed.

5% non-fat milk in PBST (0.2%) was used to block the membrane for 1 hour a room temperature.

The working concentration of the primary antibody was 1ug/mL and theprimary antibody was diluted in fresh blocking buffer [5% non-fat milk/PBST (0.2%)]. The antibody wasincubatedovernight at4℃.

It therefore seems that the blocking and diluent you have been using should not be contributing to the problem. If you could pleaseprovide the information requested inmy previous email hopefully I can try to isolate where the problem may be coming from, and if a replacement antibody may be required.

From my previous email:

I was hoping you may be able to clarify a little further the problems you have been experiencing. Do you observe no bands at all or is there high background? Would it be possible to send an image of the results obtained? I also have a few further questions:

1. how was the tissue samples prepared? How were they lysed and were any protease inhibitors used?

2. Was the sample run denatured or native? Was boiling and reducing agents employed?

3. What buffer was employed in the wash steps?

4. Do you have the order number (or purchase order number) of ab102815?

This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I was hoping you may be able to clarify a little further the problems you have been experiencing. Do you observe no bands at all or is there high background? Would it be possible to send an image of the results obtained? I also have a few further questions:

1. how was the tissue samples prepared? How were they lysed and were any protease inhibitors used?

2. Was the sample run denatured or native? Was boiling and reducing agents employed?

3. Whatbuffer was employed in the wash steps?

4. Do you have the order number (or purchase order number) of ab102815?

This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.