Anti-G3BP 抗体 [2F3] (ab56574)
Key features and details
- Mouse monoclonal [2F3] to G3BP
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-G3BP antibody [2F3]
G3BP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2F3] to G3BP -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment: KPEPVLEETA PEDAQKSSSP APADIAQTVQ EDLRTFSWAS VTSKNLPPSG AVPVTGIPPH VVKVPASQPR PESKPESQIP PQRPQRDQRV , corresponding to amino acids 214-303 of Human G3BP
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特記事項
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
2F3 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab56574の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (4) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain. -
ドメイン
The NTF2 domain mediates multimerization. -
翻訳後修飾
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS. - Information by UniProt
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参照データベース
- Entrez Gene: 10146 Human
- Omim: 608431 Human
- SwissProt: Q13283 Human
- Unigene: 587054 Human
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別名
- ATP dependent DNA helicase VIII antibody
- ATP-dependent DNA helicase VIII antibody
- G3BP antibody
see all
画像
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ICC/IF image of ab56574 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56574, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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G3BP antibody (ab56574) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymphoma.
This image was generated using the ascites version of the product.
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G3BP antibody (ab56574) at 1ug/lane + A-431 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab56574 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56574, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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ab56574 staining G3BP in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Top row - untreated cells. Bottom row - cells treated with sodium arsenite. Left - G3BP, Middle - Nucleus, Right - Merge.
Stress granules are visible in cells treated with sodium arsenite, whereas G3BP is dispersed in the cytoplasm in untreated cells.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (66)
ab56574 は 66 報の論文で使用されています。
- Somasekharan SP et al. Regulation of AR mRNA translation in response to acute AR pathway inhibition. Nucleic Acids Res 50:1069-1091 (2022). PubMed: 34939643
- Böddeker TJ et al. Non-specific adhesive forces between filaments and membraneless organelles. Nat Phys 18:571-578 (2022). PubMed: 35582428
- Vanneste J et al. Cellular Stress Induces Nucleocytoplasmic Transport Deficits Independent of Stress Granules. Biomedicines 10:N/A (2022). PubMed: 35625794
- Hallacli E et al. The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability. Cell 185:2035-2056.e33 (2022). PubMed: 35688132
- Peng G et al. Amyotrophic lateral sclerosis (ALS) linked mutation in Ubiquilin 2 affects stress granule assembly via TIA-1. CNS Neurosci Ther 28:105-115 (2022). PubMed: 34750982